FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751735
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG33260.442443148183868No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA26530.3529169188610348No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT23130.30768821459689916No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT22500.2993076017479564No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG21390.2845417600617239No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA19650.2613953055265486No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG17280.22986823814243051No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG16650.22148762529348773No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16460.2189601388787272No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC14840.19740999155287436No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14710.19568065874277502No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14660.1950155307388907No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA13970.1858367642852867No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13540.18011666345188132No Hit
GGTTAGGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13450.1789194330448895No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT12980.17266722980837662No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12680.16867646178507054No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12070.16056190013768148No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11860.15776836252136722No Hit
ATTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTG11830.15736928571903663No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC11660.15510785050582987No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11510.1531124664941768No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11420.15191523608718496No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11290.15018590327708567No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11260.14978682647475505No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT10690.1422043672304735No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA10600.14100713682348168No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG10590.1408741112227048No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10500.13967688081571297No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10180.13542006159085315No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10140.13488795918774568No Hit
CCCACATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA9960.13249349837376204No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9760.12983298635822466No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9710.1291678583543403No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9700.12903483275356342No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9500.12637432073802604No Hit
CCTTTACCCCGTTGCCCGACAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9430.12544314153258795No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA9410.1251770903310342No Hit
CATTTGAACCCCAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT9320.12397985992404238No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA9310.12384683432326551No Hit
GCCCAAAAGACCTACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9290.12358078312171177No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9040.12025514310229003No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8990.11959001509840568No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8990.11959001509840568No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAC8940.11892488709452134No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTTCTTAAACAAACAGTCTTTGA8930.11879186149374447No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8820.11732857988519892No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA8820.11732857988519892No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG8800.11706252868364517No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACCGCCGTG8650.11506714467199212No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8580.11413596546655402No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT8570.11400293986577716No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCTACAATTCT8550.11373688866422343No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8300.11041124864480169No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCTACAAT8270.11001217184247108No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8040.10695258302460307No Hit
CAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTAA8000.10642048062149562No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7910.10522325021450378No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTCAAA7900.1050902246137269No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7900.1050902246137269No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTGGGGTTCAAATGTATA7880.10482417341217318No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7820.10402601980751196No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7800.10375996860595821No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7700.10242971259818952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)