FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751735
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG38080.5065614877583191No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA27730.36887999095425916No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT24020.31952749306604056No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT21690.28853252808503No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG21470.2856059648679388No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA20920.278289556825211No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17290.2300012637432074No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG16560.22029039488649593No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15670.20845111641735453No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG15600.20751993721191642No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14980.1992723499637505No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13910.1850386106806255No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13740.1827771754674187No Hit
GGTTAGGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13680.1819790218627575No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT12830.1706718457967236No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC12660.1684104105835168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12550.16694712897497124No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12470.16588292416875627No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12450.16561687296720254No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA12410.16508477056409507No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11240.14952077527320135No Hit
ATTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTATTG11150.1483235448662095No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11140.1481905192654326No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10780.14340159763746532No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10610.14114016242425853No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10590.1408741112227048No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10420.13861267600949803No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10370.13794754800561368No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT10090.13422283118386133No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA9940.13222744717220827No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT9820.13063113996288586No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA9810.13049811436210898No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATG9610.1278376023465716No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG9450.1257091927341417No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA9440.1255761671333648No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9440.1255761671333648No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9310.12384683432326551No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9140.12158539911005872No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA9140.12158539911005872No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9010.11985606629995943No Hit
CCTTTACCCCGTTGCCCGACAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9000.11972304069918255No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8960.11919093829607509No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8900.11839278469141386No Hit
CCCACATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA8730.11613134947820708No Hit
CATTTGAACCCCAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT8700.11573227267587646No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8660.11520017027276899No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8630.11480109347043839No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8630.11480109347043839No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAC8610.11453504226888464No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACCGCCGTG8510.11320478626111595No Hit
GCCCAAAAGACCTACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8460.1125396582572316No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8230.1094800694393636No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8160.10854889023392551No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8040.10695258302460307No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7890.10495719901295003No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7690.10229668699741265No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7680.10216366139663578No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCTACAATTCT7600.10109945659042083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)