FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497415
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG27920.561301930983183No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT25340.5094337726043645No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT24480.4921443864780917No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT20660.4153473457776706No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA19150.38499040036991244No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC15550.31261622588784016No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG15540.3124151865142788No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA15540.3124151865142788No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC15260.3067860840545621No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14750.2965330760029352No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14720.29592995788225124No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14350.2884915010604827No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA14310.28768734356623743No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG14270.2868831860719922No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12710.25552104379642754No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA11690.23501502769317373No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT11630.23380879145180586No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11630.23380879145180586No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11570.232602555210438No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA11480.23079320084838617No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11240.2259682558829147No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG11060.22234954715881103No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10920.21953499592895268No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10790.2169214840726556No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10760.2163183659519717No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10750.21611732657841037No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10710.21531316908416512No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA10490.21089030286581628No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC10310.20727159414171267No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG10200.2050601610325382No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA9970.20043625544062804No Hit
GGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA9730.19561131047515656No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9620.19339987736598213No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9600.1929977986188595No Hit
GTTTTAGTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACA9440.18978116864187852No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA9200.18495622367640702No Hit
GTTTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9190.1847551843028457No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9190.1847551843028457No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8960.18013127871093554No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG8950.17993023933737423No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8930.1795281605902516No Hit
CTTTACCACAGGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA8760.1761104912397093No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8740.17570841249258667No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8720.17530633374546403No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT8650.17389905813053486No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT8600.1728938612627283No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT8460.17007931003286994No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8420.16927515253862468No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7990.16063045947548826No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7730.15540343576289417No Hit
GGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7710.15500135701577153No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7520.1511816089181066No Hit
ATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7490.15057849079742266No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7470.15017641205030005No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7340.147562900194003No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATATGGT7340.147562900194003No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7210.14494938833770596No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7110.1429389946020928No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7060.14193379773428627No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6990.14052652211935707No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACACC6940.13952132525155053No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6880.13831508901018266No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6820.13710885276881477No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6780.1363046952745695No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC6740.13550053778032428No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6710.13489741965964033No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6660.13389222279183377No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6500.13067559281485278No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6500.13067559281485278No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6490.13047455344129147No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6480.13027351406773016No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6410.12886623845280099No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6390.12846415970567834No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6380.12826312033211704No Hit
CAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6380.12826312033211704No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6370.12806208095855573No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA6280.12625272659650394No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6210.12484545098157473No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6110.12283505724596162No Hit
GTAGAGACCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6090.122432978498839No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGACCATATTGTACTAAAA6090.122432978498839No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6090.122432978498839No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6080.12223193912527769No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA6070.12203089975171637No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6050.12162882100459375No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5970.12002050601610326No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5960.11981946664254195No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5960.11981946664254195No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5950.11961842726898064No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5920.11901530914829671No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5920.11901530914829671No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5900.11861323040117405No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5800.11660283666556094No Hit
AGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGT5780.11620075791843833No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5740.11539660042419309No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG5680.11419036418282522No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5600.11258204919433472No Hit
ACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAAT5590.11238100982077341No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5330.10715398610817929No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5320.10695294673461798No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5300.10655086798749536No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5180.1041383955047596No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5120.10293215926339173No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5090.1023290411427078No Hit
GTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAA5070.10192696239558517No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5070.10192696239558517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)