FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587948
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48890.8315361222421029No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT24370.41449243810677133No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20180.34322763237565224No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC19620.33370298053569364No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18960.3224774980100281No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT17870.3039384435358229No Hit
GGATATCTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC16800.28573955519875904No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16040.27281324198738666No Hit
ATCCAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG15220.25886643036459006No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14930.25393402137604004No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG14400.24491961874179347No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14010.23828637906753658No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11810.2008681039819848No Hit
ATGTAATAGGAAGTTGGGGTACTTTACCACAGGACCATATTGTACTAAAA11600.1972963595420003No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11590.19712627647342965No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT11550.19644594419914685No Hit
GTGGTAAAGTACCCCAACTTCCTATTACATATCCCATGAAGTTAAGGGAG11270.19168361827916752No Hit
GTAGATGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG11120.1891323722506072No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10980.18675120929061753No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA10840.18437004633062787No Hit
CATTTGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT10630.1807983018906434No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10620.1806282188220727No Hit
GGCAACATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG10500.17858722199922442No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCTATTACATATCCCATGAA10320.175525726764952No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10060.17110356698211407No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10000.17008306857068992No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9570.16276949662215026No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9500.16157891514215542No Hit
CTATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA9410.16004816752501924No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT9200.15647642308503473No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9190.15630634001646404No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9050.15392517705647438No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8970.15256451250790887No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCTATTACATATCCC8890.15120384795934333No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8870.15086368182220197No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8820.1500132664793485No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8760.14899276806792436No Hit
CCCTTAACTTCATGGGATATGTAATAGGAAGTTGGGGTACTTTACCACAG8680.14763210351935885No Hit
GAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAG8660.14729193738221746No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8580.14593127283365195No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8580.14593127283365195No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8370.14235952839366747No Hit
GAGATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG8190.13929803315939504No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8120.13810745167940022No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7990.13589637178798125No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA7900.13436562417084505No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT7790.13249471041656746No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7700.13096396279943123No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7600.12926313211372434No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT7440.1265418030165933No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7350.1250110553994571No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7320.12450080619374503No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7280.12382047391946227No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7200.12245980937089673No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC7170.12194956016518467No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7130.12126922789090192No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7110.12092906175376053No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7070.12024872947947778No Hit
GGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGC7020.11939831413662431No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6920.11769748345091743No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6910.11752740038234674No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6840.11633681890235192No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6840.11633681890235192No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6740.11463598821664502No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6650.1131052405995088No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6640.11293515753093811No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6490.11038391150237775No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6480.11021382843380707No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6390.10868308081667087No Hit
GTATATTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACCAGCACG6380.10851299774810018No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6310.10732241626810535No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6200.10545150251382776No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6190.10528141944525705No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6100.10375067182812085No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6060.1030703395538381No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5920.10068917659384843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)