FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587948
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48840.8306857068992497No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT23260.3956132174954248No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20960.35649411172416606No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT20730.3525822011470402No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC19560.3326824821242695No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT17810.30291794512439874No Hit
GGATATCTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC16800.28573955519875904No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14340.24389912033036934No Hit
ATCCAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG13600.23131297325613828No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG13420.22825147802186585No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13360.22723097961044172No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT12620.2146448325362107No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12290.20903209127337793No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG12220.2078415097933831No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA11640.19797669181628308No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11490.19542544578772272No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT10880.18505037860491064No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10850.18454012939919856No Hit
ATGTAATAGGAAGTTGGGGTACTTTACCACAGGACCATATTGTACTAAAA10780.18334954791920374No Hit
CATTTGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT10630.1807983018906434No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCTATTACATATCCCATGAA10410.1770564743820882No Hit
GTAGATGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG10290.17501547755923993No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10240.1741650622163865No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA10180.17314456380496235No Hit
GTGGTAAAGTACCCCAACTTCCTATTACATATCCCATGAAGTTAAGGGAG9830.1671916564049882No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9740.16566090878785197No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9660.16430024423928646No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG9650.16413016117071577No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9640.16396007810214508No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9540.16225924741643818No Hit
GGCAACATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG9510.1617489982107261No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9460.16089858286787267No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9130.15528584160503991No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9070.15426534319361576No Hit
CCCTTAACTTCATGGGATATGTAATAGGAAGTTGGGGTACTTTACCACAG9030.153585010919333No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCTATTACATATCCC8990.15290467864505025No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT8960.15239442943933817No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8890.15120384795934333No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA8720.14831243579364162No Hit
GGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGC8700.14797226965650023No Hit
CTATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA8620.1466116051079347No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8470.14406035907937437No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8270.14065869770796055No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA8090.13759720247368815No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8060.13708695326797607No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7780.13232462734799674No Hit
GAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTTGCGAAAG7730.1314742120051433No Hit
GAGATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG7590.12909304904515367No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7540.1282426337023002No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7430.1263717199480226No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC7400.12586147074231055No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT7350.1250110553994571No Hit
GTATATTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACCAGCACG7340.1248409723308864No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7330.1246708892623157No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7230.12297005857660882No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7230.12297005857660882No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7160.12177947709661398No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7020.11939831413662431No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6990.11888806493091225No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6970.11854789879377088No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6900.11735731731377605No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6900.11735731731377605No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6670.11344540673665017No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6610.11242490832522604No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6470.11004374536523638No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6400.10885316388524154No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6280.10681216706239327No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6250.10630191785668121No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6250.10630191785668121No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6200.10545150251382776No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6100.10375067182812085No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6090.10358058875955016No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6080.10341050569097948No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5900.10034901045670706No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5900.10034901045670706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)