FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715270
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG36560.5111356550673172No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT32780.45828847847665916No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA31300.43759699134592533No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA30550.4271114404350805No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT27840.38922364981056107No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC27370.38265270457309825No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC27110.37901771359067205No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT25940.36266025416975406No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA25550.3572077676961148No Hit
CATTTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT23220.32463265619975673No Hit
AATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCC22870.3197393991080291No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA22140.30953346288814015No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT21970.3071567380150153No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG21570.301564444195898No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20950.2928963887762663No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTA20860.2916381226669649No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTA20270.28338948928376695No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC20000.27961469095586283No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA19420.2715058649181428No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG18870.26381646091685657No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA17740.24801823087785033No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT17300.24186670767682136No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16880.23599479916674823No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT16590.2319403861478882No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT16500.23068212003858682No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15900.22229367930991095No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT15630.2185188809820068No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT15570.2176800369091392No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC15360.21474408265410266No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT15190.21236735778097782No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC15060.2105498622897647No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAATAAA14170.1981070085422288No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG13220.18482531072182531No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13120.18342723726704602No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC13110.18328742992156807No Hit
CATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCA13060.18258839319417844No Hit
AATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTT12960.1811903197393991No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT12850.17965243893914187No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA12610.1762970626476715No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACAT12220.1708445761740322No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTA12050.16846785130090736No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG12030.1681882366099515No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11940.16692997050065012No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA11890.16623093377326045No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC11780.16469305297300318No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC11640.16273575013631217No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT11340.15854152977197422No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11210.15672403428076112No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA11040.15434730940763627No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTTAAATGTATA10850.15169096984355557No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA10800.15099193311616593No Hit
ATGTTATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA10770.15057251107973213No Hit
GAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10740.15015308904329833No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10280.14372195115131348No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10150.14190445566010038No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA9980.13952773078697553No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG9800.1370111985683728No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA9760.13645196918646105No Hit
GTCTCTGGGTATACATTTAAACCCTAATAAAACCAAACGCTGGGGCTACT9700.13561312511359347No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9670.13519370307715967No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9610.1343548590042921No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG9580.13393543696785828No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA9530.13323640024046865No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAA9410.13155871209473347No Hit
TAATCAATTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATAT9350.13071986802186586No Hit
CTTCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG9170.1282033358032631No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8930.12484795951179274No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC8930.12484795951179274No Hit
TAGCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCT8850.12372950074796929No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8790.12289065667510171No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT8700.12163239056580033No Hit
GTATACATTTAAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC8690.1214925832203224No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG8650.12093335383841068No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8630.12065373914745481No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATA8630.12065373914745481No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8590.12009450976554308No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8280.11576048205572721No Hit
GTTTAGATTGTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACAT8220.1149216379828596No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC8130.11366337187355822No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG8050.11254491310973477No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG8010.11198568372782305No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7750.10835069274539684No Hit
CAATAACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTA7560.10569435318131615No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA7410.10359724299914717No Hit
ATATAGTATTAGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7410.10359724299914717No Hit
CGATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT7350.1027583989262796No Hit
CAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGT7350.1027583989262796No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7280.10177974750793406No Hit
CCTTTTACACAATGTGGCTATCCCGCCTTGATGCCTTTATATGCATGTAT7270.10163994016245614No Hit
AGAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTAT7240.10122051812602234No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG7200.10066128874411061No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7180.10038167405315476No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG7170.10024186670767683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)