FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644904
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN126251.9576557130983836No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG27310.4234738813838959No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22040.34175629240941285No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT19970.3096584918065325No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15730.243912272214159No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG13260.20561199806482824No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13020.20189051393695806No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAGTTAATGAAAAA13020.20189051393695806No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12470.19336211281058888No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12170.1887102576507511No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10920.16932752781809385No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10620.1646756726582561No Hit
CTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT9900.15351122027464553No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9860.1528909729200005No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9770.1514954163720492No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9620.14916948879213032No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9620.14916948879213032No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA9040.14017590214977732No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9030.14002084031111608No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8780.1361442943445846No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8760.1358341706672621No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8700.13490379963529456No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8690.1347487377966333No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8510.13195762470073066No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8310.12885638792750548No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8190.1269956458635704No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8110.12575515115428032No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTATTACAATA7690.11924255393050748No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7460.11567613164129856No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7340.11381538957736345No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7330.11366032773870219No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7280.11288501854539591No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7090.109938843610832No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7040.10916353441752571No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7030.10900847257886445No Hit
GTTTTATTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA7010.10869834890154194No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6990.10838822522421941No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTATTACAATATGTT6940.10761291603091314No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6940.10761291603091314No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT6870.10652748316028432No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6800.1054420502896555No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6800.1054420502896555No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6760.10482180293501049No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6710.10404649374170419No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC6680.10358130822572041No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6670.10342624638705916No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC6630.10280599903241412No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6480.10048007145249525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)