FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498976
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG40920.8200795228628232No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT31440.6300904251907907No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT31310.6274850894632207No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19330.3873933816456102No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA17990.36053838260758037No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16940.3394952863464375No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC16440.32947476431732187No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA16020.3210575258128648No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA14550.2915971910472648No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA14360.28778939267620085No Hit
GGATATCTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC13560.27175655742961585No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC12970.2599323414352594No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12970.2599323414352594No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA12680.25412043865837236No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12210.24470114795100364No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA12040.24129417046110435No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA11690.2342798050407234No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11310.22666420829859552No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11130.2230568203681139No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG10240.20522029115628806No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG10130.20301577630988263No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10050.20141249278522416No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA9560.1915923811966908No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9410.18858622458795612No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9000.18036939652408132No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAAC8360.1675431283268133No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8040.1611299942281793No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7960.1595267107035208No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT7950.15932630026293848No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7940.15912588982235618No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7780.1559193227730392No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT7770.15571891233245688No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA7730.1549172705701276No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAACATTCATTTACAGG7710.154516449688963No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7440.14910536779324055No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7430.14890495735265824No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC7420.14870454691207594No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA7180.14389469633810043No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6880.13788238312063106No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAAT6880.13788238312063106No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCC6820.13667992047713717No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC6720.13467581607131404No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6710.13447540563073174No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6630.13287212210607324No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATATGTT6590.132070480343744No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6540.13106842814083242No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6330.12685980888860388No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGT6240.12505611492336305No Hit
ATCTCAATGTTAGTATCCCCTGGACTCATAAGGTGGGAAACTTTACTGGG6150.12325242095812224No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6130.12285160007695761No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6030.12084749567113448No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6010.12044667478996986No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5890.1180417495029821No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG5840.11703969730007054No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5790.11603764509715898No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5610.11243025716667736No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5520.11062656320143655No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5510.11042615276085421No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5460.10942410055794266No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5460.10942410055794266No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG5450.10922369011736036No Hit
ATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGGAT5360.10741999615211954No Hit
GAGATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG5280.10581671262746103No Hit
ATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5220.10461424998396718No Hit
GATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT5190.10401301866222024No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5190.10401301866222024No Hit
GAAAAACGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT5170.1036121977810556No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5130.10281055601872634No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5120.10261014557814403No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTG5100.10220932469697942No Hit
ATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATTTACAT5020.10060604117232091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)