FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462095
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44330.9593265454073296No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAATTTTAT25230.5459916250987351No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT19840.4293489433990846No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG19500.42199114900615675No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19250.41658100607018034No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT18180.39342559430420154No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT17550.3797920341055411No Hit
GGGTAAAGGTTCAGGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGA14110.3053484673065062No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT13900.3008039472402861No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG13130.2841407069974789No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT11640.2518962550990597No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAA11110.24042675207478983No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11010.23826269490039928No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10870.23523301485625248No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10720.2319869290946667No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC10360.2241963232668607No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10300.22289788896222637No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT10060.2177041517436891No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT10020.21683852887393285No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9940.2151072831344204No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9820.21251041452515174No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC9470.20493621441478485No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9330.20190653437063807No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGT9260.2003916943485647No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9190.19887685432649133No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9190.19887685432649133No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9050.19584717428234455No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9010.19498155141258833No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC8780.19000421991149005No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT8780.19000421991149005No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG8780.19000421991149005No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG8730.18892219132429477No Hit
CCCTAAATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8690.18805656845453858No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCAT8650.18719094558478233No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG8290.17940033975697636No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC8190.17723628258258584No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG8160.17658706543026867No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTATGCAAACAATACC7930.17160973392917042No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7880.17052770534197514No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7860.170094893907097No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7820.1692292710373408No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7500.16230428807929104No Hit
CAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTTTG7490.162087882361852No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7480.16187147664441293No Hit
ATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTT7480.16187147664441293No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT7480.16187147664441293No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC7220.1562449279909975No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAA7210.15602852227355848No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7140.1545136822514851No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCA7110.15386446509916793No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7110.15386446509916793No Hit
AATTTAGGGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA7100.15364805938172887No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT7000.15148400220733832No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6630.1434769906620933No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6630.1434769906620933No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6580.14239496207489802No Hit
GACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTTTGTTAG6400.13849965916099505No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTGCAT6280.13590279055172635No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAA6250.13525357339940922No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6200.13417154481221394No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA6130.13265670479014055No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6120.13244029907270152No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG6090.13179108192038433No Hit
GGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5920.1281121847239204No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACA5870.12703015613672514No Hit
GGGTTACTCCCTAAATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT5830.12616453326696891No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAAT5810.1257317218320908No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5800.12551531611465175No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5800.12551531611465175No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5720.12378407037513932No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC5710.12356766465770026No Hit
TCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTT5690.12313485322282215No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTT5640.12205282463562687No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCA5610.12140360748330971No Hit
CATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGT5580.12075439033099256No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT5560.12032157889611443No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5500.11902314459148011No Hit
ATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5500.11902314459148011No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5490.11880673887404104No Hit
GTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAAAT5440.11772471028684578No Hit
CCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATC5430.11750830456940672No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5420.11729189885196767No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5420.11729189885196767No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5390.1166426816996505No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5360.11599346454733335No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5330.11534424739501618No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5320.11512784167757711No Hit
CATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCA5300.114695030242699No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5260.1138294073729428No Hit
GAACACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAA5250.11361300165550374No Hit
CCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTT5230.11318019022062563No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5220.11296378450318659No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGT5200.11253097306830846No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAATTTTATGG5170.11188175591599131No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC5160.11166535019855224No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5140.11123253876367414No Hit
GTTCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAAATTT5050.10928488730672263No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAAT5040.10906848158928359No Hit
CAAGAACACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGT5030.10885207587184455No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5020.10863567015440548No Hit
TTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4960.10733723584977115No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT4910.10625520726257587No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC4830.10452396152306344No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4800.10387474437074627No Hit
GGTTCAGGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4780.10344193293586816No Hit
GATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAA4770.1032255272184291No Hit
GCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA4750.102792715783551No Hit
AGAACACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA4740.10257631006611195No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT4730.1023599043486729No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4720.10214349863123383No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA4710.10192709291379479No Hit
GGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGA4700.10171068719635572No Hit
ACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATT4680.10127787576147762No Hit
CCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCG4660.10084506432659951No Hit
ACCCAAAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTGT4650.10062865860916047No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4630.10019584717428234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)