FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462095
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44160.9556476482108658No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAATTTTAT23430.5070385959597052No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG21330.46159339529750376No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT21230.4594293381231132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20460.442766097880306No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT18180.39342559430420154No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT18150.39277637715188435No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT15910.3443014964455361No Hit
GGGTAAAGGTTCAGGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGA15210.32915309622480227No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG14310.30967658165528733No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA12440.2692087124941841No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT12190.26379856955820774No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT11730.2538439065560112No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAA11430.24735173503283958No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11150.24129237494454606No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10900.23588223200856967No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10870.23523301485625248No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC10100.21856977461344526No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9920.2146744716995423No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9870.213592443112347No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT9680.20948073448100502No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC9600.20774948874149254No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG9550.20666746015429727No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC9500.205585431567102No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9130.197578420021857No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGAATGT9120.1973620143044179No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC9100.19692920286953983No Hit
CCCTAAATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8680.18784016273709953No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8590.18589251128014803No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG8400.18178080264880597No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG8230.17810190545234206No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC8120.17572144256051245No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8120.17572144256051245No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC8070.17463941397331717No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG8010.17334097966868284No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7930.17160973392917042No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7820.1692292710373408No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT7710.1668488081455112No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7690.1664159967106331No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC7650.16555037384087687No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT7650.16555037384087687No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCAT7560.16360272238392537No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG7320.15840898516538807No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTATGCAAACAATACC7300.15797617373050996No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTTCTCGTGTTAC7220.1562449279909975No Hit
GTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAAAT7200.1558121165561194No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7070.15299884222941168No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7020.15191681364221643No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6960.1506183793375821No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6820.14758869929343532No Hit
AATTTAGGGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA6730.14564104783648385No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCA6720.1454246421190448No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAA6660.14412620781441043No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCA6640.14369339637953235No Hit
CAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTTTG6440.13936528203075124No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC6400.13849965916099505No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6380.1380668477261169No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAA6360.1376340362912388No Hit
ATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTT6330.13698481913892163No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTGCAT6320.1367684134214826No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6230.1348207619645311No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6120.13244029907270152No Hit
ATTCGTTGACACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5870.12703015613672514No Hit
GACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTTTGTTAG5780.12508250467977364No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5770.1248660989623346No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5760.12464969324489553No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5710.12356766465770026No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAAT5690.12313485322282215No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA5680.12291844750538308No Hit
GGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5670.12270204178794404No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5670.12270204178794404No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5640.12205282463562687No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACA5620.12162001320074876No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5590.1209707960484316No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5570.12053798461355349No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT5570.12053798461355349No Hit
GGGTTACTCCCTAAATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT5550.12010517317867539No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5500.11902314459148011No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC5470.11837392743916295No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5430.11750830456940672No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5340.11556065311245522No Hit
CATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCA5270.11404581309038184No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5260.1138294073729428No Hit
GTGTGTCAACGAATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACA5240.11339659593806467No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5140.11123253876367414No Hit
ACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATT5120.11079972732879603No Hit
CATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGT5120.11079972732879603No Hit
ACCCAAAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTGT5100.11036691589391791No Hit
GGTTCAGGTATTGTTTGCATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5050.10928488730672263No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAAT5010.10841926443696642No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5010.10841926443696642No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC4980.10777004728464927No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4960.10733723584977115No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG4950.1071208301323321No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTT4950.1071208301323321No Hit
GAACACATCATACAAAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAA4940.10690442441489303No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC4920.10647161298001494No Hit
TCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATCTTTTTGTTT4890.10582239582769776No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT4890.10582239582769776No Hit
GGATGTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGA4880.10560599011025872No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA4860.10517317867538059No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC4850.10495677295794155No Hit
GTTCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAAATTT4840.10474036724050251No Hit
CCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCATC4810.10409115008818534No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC4770.1032255272184291No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4690.10149428147891668No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4660.10084506432659951No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT4650.10062865860916047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)