FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425029
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA36280.8535888139397547No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14210.33433012806184986No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT13140.309155375280275No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12110.28492173475221694No Hit
GTGTATATGTACCACATTTTCTTTATCCAATCCACCATTTATGGGCATCT11980.2818631199282873No Hit
GTGCTGGTGAGACTGCAGAGAAAAAAAAATGCTTATTTTTGGTGTTAGTG11110.26139392841429643No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11010.25904114778050435No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATATTTAATCT10400.2446891859143729No Hit
GTATTTCATGGTGTATATGTACCACATTTTCTTTATCCAATCCACCATTT10130.23833667820313437No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9330.21951443313279798No Hit
CATATACACCATGAAATACTATGCGATCATAAAAGAGAATAAAAGTATTG9120.2145735938018347No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8930.2101033105976298No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8800.20704469577370013No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8580.20186857837935765No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8340.19622190485825675No Hit
GTGCTGAGGAGGTTGGGCTTCTATTGGTCCTGCCACTCAAATTGTGAACA8190.19269273390756866No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8000.18822245070336377No Hit
ATGAAATACTATGCGATCATAAAAGAGAATAAAAGTATTGTTCCTTGCAG7940.18681078232308856No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7690.18092883073860844No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7680.1806935526752292No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7550.17763493785129958No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC7410.1743410449639907No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7180.168929649506269No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7020.1651652004922017No Hit
GTGTCTATTGTTTCCATCTTTATGTACATGTGAACCCAAGATTTATCTCA6740.15857741471758396No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6710.15787158052744638No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6270.14751934573876135No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGT6210.14610767735848612No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6140.1444607309148317No Hit
CATATGTTCATTGCAGCACTATTCACAATAGCAAAGACATGGAATCAACC6070.14281378447117726No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC5970.14046100383738522No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5870.13810822320359317No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT5760.1355201645064219No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5690.13387321806276747No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5670.13340266193600905No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5580.13128515936559623No Hit
CACTTACAGTTAATGAGAAGAGAAGACTGCAATTGATTATGCCTGCTAGG5510.1296382129219418No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5470.12869710066842496No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5420.12752071035152895No Hit
GTGCTGGGAGGATTTTCCATTCTTCAAGTGAAACTGAAATTGAGACTTTT5390.12681487616139134No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5380.1265795980980121No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCTT5290.12446209552759929No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5240.12328570521070326No Hit
ATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGTTAATGAGAAGAG5180.12187403683042804No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA5160.12140348070366963No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5060.11905070006987759No Hit
GTATATGTACCACATTTTCTTTATCCAATCCACCATTTATGGGCATCTAG5020.11810958781636076No Hit
ATTCACAATAGCAAAGACATGGAATCAACCTAGATGCCCATAAATGGTGG4990.11740375362622316No Hit
CTATTGGTCCTGCCACTCAAATTGTGAACATAGGACCCAATAGAAAGGTT4980.11716847556284395No Hit
CTACAAACCTTACCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4940.11622736330932713No Hit
ACTCAAAGGAATATAAATAATTCTACCACAAAGACACATGCATGCATATG4850.11410986073891428No Hit
TTGCAGCACTATTCACAATAGCAAAGACATGGAATCAACCTAGATGCCCA4790.11269819235863905No Hit
GTTGGTGATAGTGTAAATTATTTCATCCATTGTGGAAAAACACGTGGCAA4760.11199235816850145No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC4740.11152180204174304No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4720.11105124591498464No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4660.1096395775347094No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4640.109169021407951No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG4610.10846318721781337No Hit
CAATAGGCCTGTTAACAGGCAGTTTTCTAAAACATTCTTTGATTTTCAGT4570.10752207496429655No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4480.1054045723938837No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4440.10446346014036688No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4420.10399290401360847No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4400.10352234788685007No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4340.10211067950657485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)