FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357890
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG21420.5985079214283718No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC20990.5864930565257481No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15930.4451088323227807No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA14970.41828494788901616No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13740.38391684595825537No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG12960.3621224398558216No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC11810.32998966162787446No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10350.2891950040515242No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10140.2833272793316382No Hit
GAAAAGAAGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA10000.2794154628517142No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA9380.2620917041549079No Hit
CTCATCAATGTCAATGACCGACCTTGAGGCATACTTCAAAGACTGTTTGT9140.2553857330464668No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8990.25119450110369107No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8260.2307971723155159No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8250.23051775685266424No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC8200.22912067953840565No Hit
CCTTCCTTTCCTAACATTCATTTACAGGAAGACATTATTAATAGATGTCA8060.22520886305848167No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7410.20704685797312025No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7370.20592919612171337No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7200.20117913325323422No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7200.20117913325323422No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7120.19894380955042054No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6740.1883260219620554No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6700.1872083601106485No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5950.16625220039676994No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5870.16401687669395623No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5690.1589873983626254No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5680.15870798289977367No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCTTCCTGT5620.15703149012266338No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5550.15507558188270137No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA5480.1531196736427394No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5460.15256084271703596No Hit
CTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5400.15088434993992567No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5340.1492078571628154No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5250.14669311799714996No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5210.1455754561457431No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5150.1438989633686328No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5110.14278130151722598No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4990.1394283159630054No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4920.1374724077230434No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC4900.13691357679733995No Hit
CTTTAGGGCATGGACATTGACGCGTATAAAGAATTTGGAGCTTCTGTGGA4850.1355164994830814No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4760.13300176031741598No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4710.1316046830031574No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4680.13076643661460227No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG4640.1296487747631954No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG4490.12545754282041968No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4410.12322221911760596No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4400.12294280365475427No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4310.12042806448908884No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGTGA4310.12042806448908884No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4310.12042806448908884No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4310.12042806448908884No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4260.11903098717483025No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4220.11791332532342338No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4210.11763390986057169No Hit
GCCTAAGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4190.11707507893486825No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4150.11595741708346141No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4130.11539858615775796No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCTTCCTGTAAATGAATG4100.11456033976920282No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4030.11260443152924084No Hit
ATGTAAGAGCTTGGGCAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGG4030.11260443152924084No Hit
CTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCAC4010.1120456006035374No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4010.1120456006035374No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3930.10981027690072369No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3920.10953086143787197No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT3910.10925144597502025No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3880.1084131995864651No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3860.10785436866076169No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3860.10785436866076169No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3850.10757495319790997No Hit
GACCAGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGGTGAAGC3800.10617787588365141No Hit
CATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCT3800.10617787588365141No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3780.10561904495794798No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3750.10478079856939283No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3740.10450138310654111No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3730.10422196764368939No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3710.10366313671798598No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3700.10338372125513426No Hit
GAATACAAGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAAGAGGGA3630.10142781301517227No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3630.10142781301517227No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG3620.10114839755232055No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3610.10086898208946883No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3610.10086898208946883No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG3590.1003101511637654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)