FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414754
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA16760.40409495749287533No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15150.36527676646879836No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT14590.3517747869821629No Hit
CCTCCTGCATATAGACCTCAAAATGCCCCTATCTTATCAACACTTCCGGA14070.33923723460171573No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA14060.33899612782516864No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13890.3348973126238686No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGTCCT13470.324770828008892No Hit
AGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAA13400.3230830805730626No Hit
ACGTAACCAGTATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCTGGTC12920.3115099552988036No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12670.3054822858851271No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11580.2792016472414974No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGTCTGCCGATTGGTGGAGGCAGGA11210.27028069650925607No Hit
GCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAG11160.2690751626265208No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC10860.2618419593301089No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA9880.23821349522849689No Hit
CACAATGCCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAG9780.23580242746302627No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT9380.2261581564011438No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA9310.2244704089653144No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT8750.21096842947867894No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8720.21024510914903774No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8280.19963641098096704No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCACCTCTATG8260.1991541974278729No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8250.19891309065132584No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7890.19023324669563163No Hit
ATTCCGTTGTCCTCTCCCGCAAGTATACATCATTTCCATGGCTGCTAGGC7320.1764901604324491No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7210.17383798589043145No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA7200.17359687911388438No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG7030.16949806391258432No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6990.16853363680639608No Hit
ACATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6910.1666047825940196No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6890.16612256904092546No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6830.16467592838164308No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6820.164434821605096No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGGACAACAGAAT6800.1639526080520019No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6710.16178264706307838No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6640.16009489962724893No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6630.15985379285070186No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6390.15406723021357238No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6340.15286169633083707No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6310.15213837600119587No Hit
GTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6280.1514150556715547No Hit
GGAGATAAGGGAGTAGGCTGCCTTCCTGTCTGCCGATTGGTGGAGGCAGG6100.1470751336937076No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6040.14562849303442524No Hit
GATGTATACTTGCGGGAGAGGACAACAGAATTGTCAGTCCCGATGAGTTT6000.14466406592823697No Hit
CTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACT5890.1420118913862193No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5830.14056525072693693No Hit
GTATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG5820.14032414395038986No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5770.13911861006765455No Hit
CAACAGAATTGTCAGTCCCGATGAGTTTTGCCCCAGACCTGCTGCGAGCA5770.13911861006765455No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTCGGAGCTTCTGTGGA5680.13694864907873103No Hit
GCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTT5660.1364664355256369No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5610.13526090164290158No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5590.13477868808980745No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5580.1345375813132604No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG5570.13429647453671334No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC5480.1321265135477898No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5450.13140319321814858No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5430.13092097966505445No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5410.13043876611196034No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5410.13043876611196034No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCTCAAAATGCC5410.13043876611196034No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5390.1299565525588662No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5380.12971544578231914No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5330.12850991189958383No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5240.1263399509106603No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5230.12609884413411324No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5230.12609884413411324No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5220.12585773735756617No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5200.12537552380447206No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5160.1244110966982838No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA5110.1232055628155485No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5100.12296445603900144No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5020.12103560182662493No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4960.11958896116734258No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4950.11934785439079551No Hit
GAATTCGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGCCTGACTTCTT4950.11934785439079551No Hit
ACGTCAACAGTATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCTGGTC4880.11766010695496608No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4880.11766010695496608No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4870.11741900017841901No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4850.11693678662532489No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACACTGCCACAG4840.11669567984877784No Hit
TATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG4810.11597235951913666No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG4720.11380239853021308No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA4710.11356129175366601No Hit
ACGTAAACAGTATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCTGGTC4660.11235575787093072No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4630.11163243754128954No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4630.11163243754128954No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4630.11163243754128954No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC4630.11163243754128954No Hit
ATTTACACACTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACA4540.10946247655236599No Hit
GGATATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAT4490.10825694266963068No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4460.10753362233998948No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCAC4460.10753362233998948No Hit
ATGTAATTGGGAGTTGGGGCACACTGCCACAGGAACATATTGTACAAAAA4450.10729251556344244No Hit
GTACCAACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTCTG4430.1068103020103483No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4400.10608698168070711No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4370.10536366135106594No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4360.10512255457451887No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4350.1048814477979718No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4330.1043992342448777No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC4320.10415812746833063No Hit
TTTTCAGGCCCATATTAACGTTGACATAGCTGACTACTAATTCCCTGGAT4270.10295259358559532No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4260.10271148680904825No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCCATAG4240.10222927325595414No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT4210.10150595292631294No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG4200.10126484614976587No Hit
GAAATGATGTATACTTGCGGGAGAGGACAACAGAATTGTCAGTCCCGATG4200.10126484614976587No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4190.10102373937321883No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTCGGAGCTTCTGTGG4160.10030041904357763No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4150.10005931226703058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)