FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725982
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA28810.396841794975633No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG22140.30496623883236773No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19010.2618522222314052No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18910.2604747776115661No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA18770.25854635514379143No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG17830.24559837571730428No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCCCT17200.23692047461231822No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC17000.2341655853726401No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC16760.23085971828502636No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT16210.22328377287591153No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT16080.22149309487012073No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACC15770.21722301654861967No Hit
GTATACATGCATATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAA15480.21322842715108642No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG14480.19945398095269581No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA13910.19160254661961315No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG13650.1880211906080316No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAGAGACCGCCGTGTGGTG13110.18058298966090067No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12740.17548644456749615No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCATAT12690.17479772225757664No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA12520.17245606640385022No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12150.1673595213104457No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGA11750.16184974283108947No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG11600.15978357590133088No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG11370.15661545327570106No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCCCTTACAGTTAATGAAAAA11240.15482477526991026No Hit
ACTCCGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGG11210.15441154188395856No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT10890.15000371910047358No Hit
GTTACTCATTACTTCAAAACCAGGCATTATTTACATACTCTGTGGAAGGC10880.14986597463848966No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10610.1461468741649242No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10600.1460091297029403No Hit
CTCCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10140.1396728844516806No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG9980.13746897305993813No Hit
GGCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAA9940.1369179952120025No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9920.13664250628803468No Hit
GCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAA9870.13595378397811517No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT9870.13595378397811517No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACTGAGT9700.13361212812438875No Hit
GGGCACATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATC9570.13182145011859797No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC9550.13154596119463016No Hit
CCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTC9530.13127047227066235No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA9440.1300307721128072No Hit
GGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTT9430.1298930276508233No Hit
GGATTGAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC9390.1293420498028877No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9330.12851558303098423No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTT9270.1276891162590808No Hit
CTGTAAGGGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8990.12383227132353143No Hit
GAGCCTGAGGGCTCCACCCCAAGAGACCGCCGTGTGGTGGGGTGAACCCT8840.12176610439377286No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC8840.12176610439377286No Hit
CTTCAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8790.12107738208385332No Hit
TTCCCTGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8740.1203886597739338No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC8730.1202509153119499No Hit
GAACCGGGGTGAACCCTGGTCCGAATGCTCCCGCTCCTACCTGATTTGCC8710.11997542638798207No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8710.11997542638798207No Hit
CCCCAAGAGACCGCCGTGTGGTGGGGTGAACCCTGGTCCGAATGCTCCCG8590.11832249284417519No Hit
CATTTGAACCCTAATAAAACAAAGCGGTGGGGCTACTCCCTTAACTTCAT8590.11832249284417519No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8570.1180470039202074No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT8500.11708279268632005No Hit
CCGATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAG8500.11708279268632005No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC8440.1162563259144166No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC8440.1162563259144166No Hit
GGTTAGGATAGAATCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8410.1158430925284649No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG8400.115705348066481No Hit
ATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGG8400.115705348066481No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG8360.11515437021854535No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8280.11405241452267412No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8280.11405241452267412No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG8060.11102203635902819No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC8050.11088429189704428No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8050.11088429189704428No Hit
CTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC8050.11088429189704428No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7860.10826714711935005No Hit
CCCCACCACACGGCGGTCTCTTGGGGTGGAGCCCTCAGGCTCAGGGCATA7810.10757842480943053No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA7710.10620098018959147No Hit
GGCCTATACTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCTTGT7660.10551225787967194No Hit
GCCTTAAAGCCTTTATATGCATGTATACAATCTAAACAGGCTTTCACTTT7660.10551225787967194No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7660.10551225787967194No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7660.10551225787967194No Hit
GGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAA7580.10441030218380071No Hit
ATACTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCTTGTTCCGA7560.10413481325983288No Hit
GAACATCAACTACCAGCACGGGACCATGCAAGACCTGCACGACTCCTGCT7550.10399706879784898No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7490.10317060202594554No Hit
GGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT7480.10303285756396163No Hit
TCCTCAGGCCATGCATTGGAATTCCACAACATTCCACCAAGCTCTGCTAG7450.10261962417800992No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7340.10110443509618695No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7290.10041571278626743No Hit
ACTCAGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGG7280.10027796832428353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)