FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473401
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27610.5832264824113172No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16630.3512878088554946No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA14800.3126313632628575No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13220.27925585286047133No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC12720.2686939824799694No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT12510.2642579969201586No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12410.26214562284405823No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12320.2602444861755679No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11930.2520062272787763No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG11640.2458803424580852No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA11590.24482415542003505No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA11420.24123311949066437No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11310.23890950800695396No Hit
CCTCTCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10940.2310937239253825No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10810.228347637626452No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10660.2251790765123014No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10450.22074309095249062No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10300.21757452983834003No Hit
GTCTTATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCC10260.21672958020789987No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9670.20426657315890756No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9600.2027879113056373No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9500.20067553722953688No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9290.1962395516697261No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9100.19222604092513534No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT8970.18947995462620484No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8930.1886350049957647No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8910.18821253018054462No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8860.1871563431424944No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCA8800.1858889186968342No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGAGGTG8640.18250912017507356No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8610.18187540795224347No Hit
GTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAACATTCA7950.16793373904998088No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7900.1668775520119307No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7850.1658213649738805No Hit
CACATATTGTTGACATCTATTAATAATATCCTCCTGTAAATGAATGTTAG7800.1647651779358303No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT7720.16307527867494997No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7680.16223032904450985No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTCAT7610.16075166719123957No Hit
ACACCTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7500.15842805570752913No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7490.1582168182999191No Hit
GTAAAATTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCC7330.15483701977815847No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7090.14976732199551754No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7070.14934484718029745No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6930.14638752347375691No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6900.1457538112509268No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6760.14279648754438626No Hit
CTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGGA6750.14258525013677623No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6710.14174030050633607No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6680.14110658828350595No Hit
TACCTCGGTAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGG6620.13983916383784573No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6600.13941668902262566No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG6480.13688184013130517No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6470.13667060272369513No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6460.1364593653160851No Hit
ATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACACAGAA6450.13624812790847507No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC6400.13519194087042485No Hit
CCCAGGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6380.1347694660552048No Hit
CACAAATTGTTGACATCTATTAATAATATCCTCCTGTAAATGAATGTTAG6320.13350204160954454No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6310.1332908042019345No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6240.13181214234866426No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6240.13181214234866426No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAACATTCATTTACAGG6200.13096719271822407No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6000.12674244456602332No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5990.12653120715841326No Hit
GATCCTTGGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGG5860.12378512085948278No Hit
GCTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGG5850.12357388345187273No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5840.12336264604426268No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5830.12315140863665265No Hit
ACCGAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5800.12251769641382253No Hit
GGAATTAATTTGGAAGACCCAGCATCCAGGGAATTAGTAGTCAGCTATGT5740.1212502719681623No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT5700.12040532233772215No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5680.11998284752250206No Hit
GAATTAATTTGGAAGACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTC5660.11956037270728198No Hit
TGGTCGGCCGGAACGGCAGATGAAGAAGGGGACGGTAGAGTCCCAAACGG5650.11934913529967194No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5620.11871542307684184No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5600.11829294826162175No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5570.11765923603879164No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5560.1174479986311816No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT5540.11702552381596151No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5510.11639181159313142No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5450.11512438714747118No Hit
CTTTACCACAGGACCATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA5420.11449067492464106No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5400.11406820010942098No Hit
GGACCATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA5380.11364572529420089No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAAC5350.11301201307137078No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5330.1125895382561507No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5300.11195582603332058No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5290.11174458862571056No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5290.11174458862571056No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5270.11132211381049047No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5260.11111087640288043No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5250.11089963899527039No Hit
AAATAAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA5250.11089963899527039No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5250.11089963899527039No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT5220.11026592677244028No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5210.11005468936483025No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG5170.10920973973439008No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG5160.10899850232678003No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5130.10836479010394993No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5110.10794231528872984No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5100.10773107788111982No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT5060.10688612825067965No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5050.10667489084306961No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5040.10646365343545958No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5030.10625241602784953No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5010.10582994121262947No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5000.10561870380501942No Hit
ATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4990.10540746639740939No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC4960.10477375417457928No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4950.10456251676696923No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4910.10371756713652908No Hit
CCTCGGTAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGGAT4910.10371756713652908No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA4910.10371756713652908No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4850.10245014269086884No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4780.10097148083759856No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4770.10076024342998853No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4750.10033776861476844No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4740.10012653120715842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)