FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432993
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA23730.548045811364156No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15820.36536387424277067No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG14440.33349268925825587No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA14090.3254094177042123No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC13150.3037000598162095No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG12200.28175975131237685No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT11980.27667883776412094No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC11720.2706741217525456No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11690.2699812699050562No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11080.2558932823394373No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10800.24942666509620248No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT10580.24434575154794652No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9340.2157078751850492No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT9090.20993410978930377No Hit
ACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCG9030.20854840609432485No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACTGAGT8900.20554604808853721No Hit
CCTATTACATAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACA8840.20416034439355832No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8430.19469136914453583No Hit
GTTTTCGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8380.19353661606538675No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8350.19284376421789728No Hit
TACCGTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8200.18937950498045003No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT8070.18637714697466243No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8070.18637714697466243No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8040.18568429512717297No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8030.18545334451134313No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACATAACCT7690.17760102357312935No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC7560.17459866556734174No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG7460.17228915940904357No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7320.16905585078742613No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7180.1658225421658087No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7150.16512969031831923No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7140.16489873970248942No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7120.16443683847082977No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7040.16258923354419125No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7040.16258923354419125No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6970.16097257923338254No Hit
GCACTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA6950.1605106780017229No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG6940.16027972738589308No Hit
CAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTGATTGGAAAG6920.15981782615423346No Hit
ACAAAAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAG6920.15981782615423346No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6910.15958687553840362No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6890.159124974306744No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGC6860.15843212245925453No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6670.154044060758488No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6650.15358215952682838No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6550.1512726533685302No Hit
CTCCAAAAGATACCAAGTACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6540.1510417027527004No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6510.15034885090521094No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6500.1501179002893811No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG6370.1471155422835935No Hit
GACAAAAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA6350.14665364105193388No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC6290.14526793735695495No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6210.1434203324303164No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6210.1434203324303164No Hit
GTACGAAAACTCAAGGAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5920.13672276457125174No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5920.13672276457125174No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5910.13649181395542193No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5840.1348751596446132No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5780.13348945594963427No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5770.13325850533380448No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5670.13094899917550631No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA5620.1297942460963572No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5540.12794664116971866No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5540.12794664116971866No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5520.12748473993805903No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5520.12748473993805903No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT5470.12632998685890995No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5460.12609903624308014No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5400.12471333254810124No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5400.12471333254810124No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG5370.1240204807006118No Hit
CCTTTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGT5300.12240382638980306No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5290.12217287577397326No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5210.12032527084733471No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCTAGCAGCC5210.12032527084733471No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5200.12009432023150489No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5110.11801576468903656No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5100.11778481407320673No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA5080.1173229128415471No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5010.11570625853073836No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4990.11524435729907874No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA4980.11501340668324891No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4950.11432055483575948No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4940.11408960421992966No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4930.11385865360409983No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4930.11385865360409983No Hit
CAACTGAGTTGTCGGTTCCGATAAGGTTCGCTCCAGACCGGCTGCGAGCA4920.11362770298827002No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4900.11316580175661037No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4870.11247294990912095No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA4830.11154914744580166No Hit
CCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGT4830.11154914744580166No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4820.11131819682997184No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4800.11085629559831221No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4740.1094705919033333No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4730.10923964128750349No Hit
ATCCTGAACATACAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4720.10900869067167367No Hit
CAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG4680.10808488820835442No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4670.1078539375925246No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4660.10762298697669477No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4640.10716108574503513No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4600.10623728328171587No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4590.10600633266588605No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4580.10577538205005622No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4570.10554443143422643No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTG4570.10554443143422643No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4550.10508253020256679No Hit
TACATACCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC4530.10462062897090714No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4500.10392777712341769No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4490.10369682650758788No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4490.10369682650758788No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4420.10208017219677916No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4390.10138732034928973No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4340.10023256727014061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)