FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432993
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17310.3997755160014134No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15180.3505830348296624No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13260.3062405165903375No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12140.280374047617398No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG11900.27483123283748234No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC11590.26767176374675805No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC11090.25612423295526715No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10750.2482719120170534No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT10730.24781001078539375No Hit
CTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT9710.2242530479707524No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9500.21940308503832626No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9480.2189411838066666No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9140.21108886286845285No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8770.2025436900827496No Hit
ACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCG8620.19907943084530236No Hit
GTTTTCGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8390.19376756668121656No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8250.19053425805959912No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACTGAGT8140.18799380128547113No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8050.18591524574300278No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT7920.18291288773721515No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7880.1819890852738959No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT7880.1819890852738959No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7840.1810652828105766No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7730.17852482603644862No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG7540.17413676433568212No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7370.17021060386657522No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7370.17021060386657522No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT7300.1685939495557665No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC7260.1676701470924472No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7110.16420588785499998No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6990.16143448046504216No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6990.16143448046504216No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG6840.1579702212275949No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6710.15496786322180728No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6710.15496786322180728No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6650.15358215952682838No Hit
CTCCAAAAGATACCAAGTACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6540.1510417027527004No Hit
CAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTGATTGGAAAG6460.14919409782606186No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6390.14757744351525315No Hit
GCACTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA6300.1454988879727848No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6290.14526793735695495No Hit
TACCGTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6230.14388223366197606No Hit
CCTTTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGT6200.14318938181448662No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6180.14272748058282697No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6100.14087987565618842No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6090.1406489250403586No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6040.13949417196120953No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACATAACCT6030.13926322134537972No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5870.13556801149210265No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5760.13302755471797464No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5750.13279660410214486No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5670.13094899917550631No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG5640.13025614732801685No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5530.12771569055388887No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5460.12609903624308014No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5410.12494428316393105No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5380.12425143131644159No Hit
GTACGAAAACTCAAGGAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5320.12286572762146271No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5270.12171097454231362No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5240.12101812269482418No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5180.11963241899984527No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA5170.11940146838401544No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5160.11917051776818563No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG5140.11870861653652598No Hit
ACAAAAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAG5110.11801576468903656No Hit
GACAAAAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA5060.11686101160988745No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5050.11663006099405765No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5040.11639911037822782No Hit
CCTATTACATAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACA5030.11616815976239801No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5000.11547530791490856No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4970.1147824560674191No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4960.11455150545158928No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4940.11408960421992966No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4930.11385865360409983No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4920.11362770298827002No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA4910.11339675237244021No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4910.11339675237244021No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4890.11293485114078057No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4840.11178009806163149No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4820.11131819682997184No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA4760.10993249313499295No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4750.10970154251916313No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT4730.10923964128750349No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4710.10877774005584387No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGC4690.10831583882418422No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA4690.10831583882418422No Hit
CCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGT4680.10808488820835442No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4680.10808488820835442No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4630.10693013512920534No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4620.10669918451337551No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4600.10623728328171587No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4560.1053134808183966No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4520.10438967835507734No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA4490.10369682650758788No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4470.10323492527592826No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4450.10277302404426861No Hit
CAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG4450.10277302404426861No Hit
ACTCAGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGG4450.10277302404426861No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4430.10231112281260898No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4380.1011563697334599No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4360.10069446850180026No Hit
CCCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC4360.10069446850180026No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4350.10046351788597044No Hit
CAACTGAGTTGTCGGTTCCGATAAGGTTCGCTCCAGACCGGCTGCGAGCA4330.10000161665431082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)