FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629301
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA25390.4034635254035827No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC25110.3990141442648271No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC23410.3720000444938114No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTCCCGTG16850.26775740067153875No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT14630.23248016449997697No Hit
GTATACATGCATATAAAGGCATTAACGCAGGATATCCACATTGTGTAAAA13660.21706623698357383No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13140.20880310058302784No Hit
GGATAATAGGGTTTGATGCCTTTATCTAAGGGCAAGTATTTGGTAACATT12610.20038105771324055No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG12500.19863308655158662No Hit
ATATGTTCCTGTGGCAAGGATCCCCAACTCCCAATTACATATCCCATGAA12130.19275354718965965No Hit
CATTTAAACCCTCACAAAACCAAACGATGGGGATACTCCCTTAATTTCAT11910.1892576048663517No Hit
AGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAA11880.1887808854586279No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT11020.17511492910387874No Hit
TCCTGAAACTGCTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAA10870.1727313320652597No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10400.16526272801092004No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGGCCCACAAT10130.16097225334140577No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10000.15890646924126928No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9940.15795303042582168No Hit
CCAGCAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGAAG9940.15795303042582168No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9720.15445708810251374No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGATCCTTGCCACAG9700.1541392751640312No Hit
ATACAGCACTCAGGCAAGCTGTTGTGTGTTGGGGTGAGTTGATGAATCTA9560.15191458459465343No Hit
AATTAAGGGAGTATCCCCATCGTTTGGTTTTGTGAGGGTTTAAATGTATA9490.15080223930996453No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9490.15080223930996453No Hit
GTTGATAGGTGTCAGCAATTTGTGGGACCCCTAACAGCAAATGAAACCAG9450.15016661343299947No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9450.15016661343299947No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9280.1474652034558979No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC9220.14651176464045026No Hit
GTGGATATCCTGCGTTAATGCCTTTATATGCATGTATACAAGCTAAACAG9170.14571723229424394No Hit
GTATGGATCGGCAGAGGAGACCGAAAGGTTCCGCGCATGCGCTGATGGCC9100.14460488700955504No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGGCCCACAATTCG9070.14412816760183123No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG8860.1407911317477646No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTT8830.14031441234004077No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCCCAAAATGCC8830.14031441234004077No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8750.13904316058611063No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGGCCCACAATTCGTTGACACACT8640.13729518942445665No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT8600.1366595635474916No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8580.13634175060900905No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8570.13618284413976778No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT8540.13570612473204396No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC8530.1355472182628027No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8440.13411706003963128No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT8380.13316362122418365No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGC8330.1323690888779773No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCA8280.13157455653177097No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT8230.13078002418556461No Hit
ATGTGGATATCCTGCGTTAATGCCTTTATATGCATGTATACAAGCTAAAC8140.1293498659623932No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCT8090.12855533361618685No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8090.12855533361618685No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG8090.12855533361618685No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8070.1282375206777043No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATT8040.1277608012699805No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8010.1272840818622567No Hit
TCACCATACAGCACTCAGGCAAGCTGTTGTGTGTTGGGGTGAGTTGATGA7820.12426485894667257No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7780.1236292330697075No Hit
ACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACG7630.12124563603108847No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7460.11854422605398689No Hit
CTTTTACACAATGTGGATATCCTGCGTTAATGCCTTTATATGCATGTATA7410.11774969370778055No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCT7300.11600172254612658No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACAG7300.11600172254612658No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCATTTATGCC7280.11568390960764405No Hit
ACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA7250.11520719019992023No Hit
GTTTACAGGAAGTTTTCTAAAACATAATTTGAGTTTTTGTACAATATGTT7250.11520719019992023No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7240.11504828373067896No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7230.1148893772614377No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7200.11441265785371388No Hit
ACCAAATACTTGCCCTTAGATAAAGGCATCAAACCCTATTATCCAGAGCA7190.11425375138447262No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGT7160.11377703197674881No Hit
ATGTAATTGGGAGTTGGGGATCCTTGCCACAGGAACATATTGTACAAAAA7140.11345921903826625No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT7100.1128235931613012No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGACGCGGTGA7000.1112345284688885No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6960.11059890259192341No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6920.10996327671495834No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATAATTTGAGTTTTTGT6860.10900983789951073No Hit
GGATTAAAGTTAGGTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC6860.10900983789951073No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6830.10853311849178691No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6780.10773858614558057No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC6690.10630842792240915No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6660.10583170851468533No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6650.10567280204544408No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6630.10535498910696155No Hit
CCCAATTACATATCCCATGAAATTAAGGGAGTATCCCCATCGTTTGGTTT6630.10535498910696155No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC6600.10487826969923773No Hit
GGAACATATTGTACAAAAACTCAAATTATGTTTTAGAAAACTTCCTGTAA6600.10487826969923773No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG6500.10328920500682504No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6490.10313029853758375No Hit
CCTCCTGCATATAGACCCCAAAATGCCCCTATCTTATCAACGCTTCCGGA6480.1029713920683425No Hit
GGCCGAGGCAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC6460.10265357912985996No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6450.10249467266061868No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6420.10201795325289488No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTCAATC6350.100905607968206No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC6350.100905607968206No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGTTCTTCTGCGAC6320.10042888856048218No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG6320.10042888856048218No Hit
GGTCCTAGGAGTCCTGATGTGATGTTCTCCATGTTCGGTACAGGGTCCCC6300.10011107562199965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)