FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621567
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26010.41845850889767316No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC23290.37469814195412565No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT17220.27704173484113537No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT16840.2709281541651986No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15960.256770388389345No Hit
CCTCTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT15230.24502587814346644No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT14590.2347293212155729No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG14480.2329596004935912No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC14350.23086811236761284No Hit
ACACTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTC13860.22298481096969433No Hit
ATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC13530.21767564880374926No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT13320.21429709106178416No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG13160.2117229518298108No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT12810.20609202225986903No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA12310.1980478371599522No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11380.1830856528741069No Hit
CTTATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCA10700.17214556113822No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10670.171662910032225No Hit
CTATTAATAATATCTTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG10400.16731905007826992No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT10170.16361872493230817No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9840.15830956276636307No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9760.1570224931503764No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGTAAAG9720.15637895834238305No Hit
TGTTTGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGGCTCCGAATGCC9540.153483051706413No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT9220.14833477324246622No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9170.14753035473247453No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9120.14672593622248287No Hit
TATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATG9060.14576063401049283No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA8960.14415179699050945No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT8910.1433473784805178No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8830.1420603088645311No Hit
CCCGCGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC8750.1407732392485444No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG8630.13884263482456435No Hit
CACTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG8620.13868175112256603No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8620.13868175112256603No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8610.13852086742056768No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8410.13530319338060098No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC8360.13449877487060927No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8310.1336943563606176No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8080.12999403121465586No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8040.12935049640666252No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8020.12902872900266585No Hit
GAATTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGAT7990.12854607789667083No Hit
AGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC7990.12854607789667083No Hit
ACCCCAACAAGGATCATTGGCCAGCGGCAAACCAGGTAGGAGTGGGATCA7960.12806342679067584No Hit
GCACCGACAACTCTGTTGTCCTTTCTCGGAAATACACCTCCTTTCCATGG7870.1266154734726908No Hit
GGTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC7780.12516752015470578No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7780.12516752015470578No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCACTTTTCCTCAC7730.12436310164471408No Hit
GTATAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC7700.12388045053871907No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG7630.12275426462473073No Hit
TGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCT7580.12194984611473902No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTATAATATGTT7560.12162807871074237No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7560.12162807871074237No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7460.12001924169075899No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7340.11808863726677896No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7250.11664068394879393No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7230.11631891654479726No Hit
GGCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGT7160.1151927306308089No Hit
GTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC7020.1129403588028322No Hit
TAGTCAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAG7010.11277947510083386No Hit
GATCATTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCAGTAAATACAC6980.11229682399483884No Hit
GATTCACTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGT6870.11052710327285716No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT6700.10779208033888543No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT6700.10779208033888543No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGTACT6650.10698766182889374No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6590.10602235961690372No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6530.1050570574049137No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6520.10489617370291537No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6520.10489617370291537No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT6450.10376998778892702No Hit
ATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTAT6440.10360910408692868No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6360.10232203447094199No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6300.10135673225895198No Hit
GATCATCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACACAGTCTTTGA6290.10119584855695363No Hit
CATCGAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT6260.10071319745095862No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6220.10006966264296528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)