FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621567
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21040.3384993090044999No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT17280.2780070370531254No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT16350.2630448527672801No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC15870.25532243507136No Hit
CCTCTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT15190.2443823433354731No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG14650.23569462342756292No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT13620.21912360212173426No Hit
ACACTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTC13150.21156206812781245No Hit
AGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC12750.20512672004787902No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG12440.20013932528593056No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT12330.19836960456394886No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA11600.18662509431807028No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11140.17922444402614682No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11090.17842002551615513No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10950.17616765368817844No Hit
GCACCGACAACTCTGTTGTCCTTTCTCGGAAATACACCTCCTTTCCATGG10820.17407616556220004No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGTAAAG10790.17359351445620502No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT10790.17359351445620502No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT10600.17053672411823664No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC9760.1570224931503764No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9590.15428747021640465No Hit
CTATTAATAATATCTTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG9530.15332216800441464No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9530.15332216800441464No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9470.15235686579242463No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9250.14881742434846124No Hit
TGTTTGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGGCTCCGAATGCC9110.14656505252048452No Hit
CCCGCGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC9080.1460824014144895No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8930.14366914588451446No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGTGGGAGTTTG8620.13868175112256603No Hit
GGTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC8610.13852086742056768No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGTACT8550.13755556520857767No Hit
ATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC8530.137233797804581No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8490.13659026299658766No Hit
GTATAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC8450.1359467281885943No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8370.13465965857260762No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8350.13433789116861095No Hit
GAATTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGAT8260.13288993785062592No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8150.13112021712864422No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8080.12999403121465586No Hit
GTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC7810.12565017126070077No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7780.12516752015470578No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7780.12516752015470578No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTATAATATGTT7660.12323691573072573No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7610.12243249722073404No Hit
CTTATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCA7580.12194984611473902No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7480.12034100909475567No Hit
CACTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG7340.11808863726677896No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7240.1164798002467956No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7220.11615803284279892No Hit
GGCACAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGT7190.1156753817368039No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCACTTTTCCTCAC7160.1151927306308089No Hit
GATTCACTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGT6820.10972268476286548No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC6800.10940091735886878No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6730.10827473144488045No Hit
ACCCCAACAAGGATCATTGGCCAGCGGCAAACCAGGTAGGAGTGGGATCA6640.10682677812689542No Hit
TAGTCAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAG6630.10666589442489707No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG6570.10570059221290705No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6560.10553970851090871No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6560.10553970851090871No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6510.10473529000091704No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6510.10473529000091704No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCG6500.10457440629891869No Hit
ATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTCTACCGTCCCCTT6500.10457440629891869No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6480.10425263889492203No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT6430.10344822038493034No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT6380.10264380187493866No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6360.10232203447094199No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT6310.10151761596095031No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6280.1010349648549553No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6260.10071319745095862No Hit
TGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCT6250.1005523137489603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)