FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358792
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA23160.6454993422372851No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16890.47074628196838275No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13260.3695734576021762No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12290.3425382951682312No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10100.2815001449307677No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9460.2636625119846596No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9160.2553011215411715No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC9040.2519565653637762No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8930.2488907222011639No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT8350.2327253673437535No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA8060.22464268991504827No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8020.2235278378559165No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA8010.22324912484113357No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7630.21265803027938193No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7360.20513277888024262No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTCT7350.20485406586545968No Hit
GTTAGCATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC7180.20011594461414972No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7020.1956565363776227No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6910.19259069321501038No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA6770.18868871100804924No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6630.1847867288010881No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCGAGATATTGGATCTTC6580.1833931637271734No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6440.1794911815202123No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6250.17419563423933643No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6230.17363820820977058No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6130.1708510780619412No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6040.16834266092889474No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6020.16778523489932887No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5890.16416196570715066No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA5740.15998127048540658No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5700.15886641842627483No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5680.15830899239670898No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5650.15747285335236014No Hit
GGACATCCTTTGTTTACGTCCCGTCGACGCTGAATCCTGCGGACGACCCT5640.1571941403375772No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5610.15635800129322838No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5540.1544070101897478No Hit
GTGTAAATGTATCTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5530.15412829717496487No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5480.15273473210105018No Hit
CTTACAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC5420.15106245401235258No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5260.14660304577582556No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5250.14632433276104262No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5150.14353720261321323No Hit
CAATAACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG5050.14075007246538385No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5030.14019264643581797No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5010.1396352204062521No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATT5010.1396352204062521No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4940.13768422930277152No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4910.1368480902584227No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4910.1368480902584227No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGTACG4900.13656937724363977No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4900.13656937724363977No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4890.13629066422885683No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4790.13350353408102747No Hit
GTTTACGTCCCGTCGACGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG4720.13155254297754687No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTGGGCGA4700.13099511694798102No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4690.13071640393319806No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4670.13015897790363218No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4630.12904412584450042No Hit
GGATAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCGGC4580.12765056077058576No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACATCCTTTGTTTA4530.12625699569667106No Hit
GCTTAGATTGAATACATGCATATAAAGGCATTAAAGCAGGATAACCACAT4510.12569956966710516No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4510.12569956966710516No Hit
GTTCTATCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGC4470.12458471760797342No Hit
ATGTATATTTGCGGGATAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4450.12402729157840754No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4430.12346986554884166No Hit
CATTTGAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT4420.12319115253405873No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4400.12263372650449286No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4360.12151887444536111No Hit
GTACAAAGACCTTTAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4360.12151887444536111No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4360.12151887444536111No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4330.12068273540101228No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4320.12040402238622935No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGATAGAACAACAGAGT4300.11984659635666348No Hit
CTGTTGTTCTATCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGT4270.11901045731231466No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4260.11873174429753172No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4250.11845303128274877No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG4170.11622332716448527No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4160.11594461414970232No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4140.11538718812013646No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4130.11510847510535352No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG4130.11510847510535352No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4130.11510847510535352No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4080.11371491003143884No Hit
CTTTCGGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT4040.11260005797230709No Hit
GCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC4010.11176391892795826No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4010.11176391892795826No Hit
CTTCAAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA3990.11120649289839239No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3960.11037035385404358No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAAGGCTCCAG3920.10925550179491181No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3900.10869807576534594No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3890.10841936275056299No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3890.10841936275056299No Hit
ATACCGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3880.10814064973578005No Hit
GGCTTTCACTTTCTCGCCCACTTACAAGGCCTTTCTGTGTAAACAATATC3870.10786193672099713No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC3850.10730451069143124No Hit
GAACAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCGGCTGCGA3840.1070257976766483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3790.10563223260273362No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3770.10507480657316774No Hit
GATTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTG3760.10479609355838479No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3740.10423866752881893No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT3720.10368124149925306No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3720.10368124149925306No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3690.10284510245490423No Hit
CCTCTAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3660.10200896341055542No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3660.10200896341055542No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3650.10173025039577248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)