FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506457
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA25080.4952049236164176No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT18020.3558051325186541No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC17640.34830202761537504No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG17000.3356652193572208No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC15960.3151304059377203No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA15240.3009139966472968No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15110.29834714496985926No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC15090.29795224471179194No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15070.2975573444537246No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC14090.278207231808426No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13970.27583783026002207No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA13740.27129647729224793No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT11830.23358350264681896No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11830.23358350264681896No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC10870.21462829025958768No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10710.21146908819504912No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT10190.20120168148529885No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT10010.197647579162693No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC9950.196462878388491No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9640.1903419243884476No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9540.188367423098111No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC9200.18165411871096657No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC9030.17829746651739437No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC8920.1761255150980241No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT8910.17592806496899044No Hit
CATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAAATTTTAT8910.17592806496899044No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC8900.1757306148399568No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT8820.17415101380768752No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8740.17257141277541824No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC8700.17178161225928362No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8660.17099181174314898No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8490.16763515954957678No Hit
GTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC8490.16763515954957678No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8470.16724025929150946No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT8440.16664790890440845No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA8240.16269890632373527No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT8230.16250145619470163No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA8150.16092185516243235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7920.15638050219465818No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7860.15519580142045622No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA7810.15420855077528794No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7760.15322130013011964No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT7750.15302385000108598No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA7740.15282639987205232No Hit
GGGTATACATTTAAACCCTAACAAAACAAAGCGATGGGGTTATTCTCTAA7720.152431499613985No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7700.15203659935591768No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7610.15025954819461476No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7550.1490748474204128No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7470.1474952463881435No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAAACCCAGCCCGAATGCTCCAG7460.14729779625910985No Hit
GTCTCCACCTTTGCGAAAAACTCATCCTCAGGCCATGCAGTGGAACTCCA7460.14729779625910985No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC7430.14670544587200887No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7380.14571819522684057No Hit
GATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC7380.14571819522684057No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTT7160.14137429238810006No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG7060.1393997910977635No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7050.13920234096872983No Hit
GACGGGGAGGCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG7040.13900489083969617No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC7040.13900489083969617No Hit
ATACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC6900.13624058903322492No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6850.13525333838805664No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6790.13406863761385468No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6740.13308138696868638No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA6710.1324890365815854No Hit
GAATAACCCCATCGCTTTGTTTTGTTAGGGTTTAAATGTATACCCAGAGA6700.13229158645255173No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6690.13209413632351807No Hit
CTCTGGGATCTTGCAGAGTTTGATGGAAGGTTGTGGAGTTCCACTGCATG6680.1318966861944844No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG6650.13130433580738346No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG6630.13090943554931614No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC6590.1301196350331815No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC6550.12932983451704685No Hit
GATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6500.12834258387187855No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC6370.125775732194441No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6340.12518338180734No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGATCAT6280.12399868103313806No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA6270.1238012309041044No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6270.1238012309041044No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA6230.12301143038796974No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT6230.12301143038796974No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6230.12301143038796974No Hit
GGATTCATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCC6170.12182672961376781No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6170.12182672961376781No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6140.12123437922666683No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG6120.12083947896859949No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCACTTATCGTCAATCTTCTCG6110.12064202883956585No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT6080.12004967845246486No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC6000.1184700774201956No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5970.11787772703309463No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5950.1174828267750273No Hit
GTATTATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGGCCTCCGTGAGGT5940.11728537664599363No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC5860.11570577561372437No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5800.11452107483952241No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5790.11432362471048875No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCAGCC5640.11136187277498386No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5620.11096697251691653No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5620.11096697251691653No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5610.11076952238788287No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5580.1101771720007819No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5560.1097822717427146No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5560.1097822717427146No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5550.10958482161368091No Hit
GTTTGATGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTTTT5540.10938737148464726No Hit
GTCCCAAGCGACCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGAGGGG5500.10859757096851264No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5500.10859757096851264No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5490.10840012083947898No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5490.10840012083947898No Hit
GTTTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG5480.10820267071044531No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5480.10820267071044531No Hit
CCGACCACTGCCTCTCACTTATCGTCAATCTTCTCGAGGATTGGGGACCC5420.10701796993624335No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC5400.10662306967817603No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5400.10662306967817603No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5400.10662306967817603No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG5340.10543836890397407No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5320.10504346864590676No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5290.10445111825880579No Hit
GTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTTGC5220.10306896735557017No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5170.10208171671040188No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA5160.1018842665813682No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5140.10148936632330088No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5140.10148936632330088No Hit
GCCTGAGGATGAGTTTTTCGCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5110.10089701593619992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)