FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002489756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135991
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7380.5426829716672427No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6650.48900294872454797No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5860.4309108691016317No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5380.3956144156598599No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC4410.32428616599627913No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3740.2750181997338059No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA3610.26545874359332605No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3560.26178202969314146No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3390.24928120243251392No Hit
GTATAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCCGGTC3010.22133817679111117No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2800.205895978410336No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2790.20516063563029907No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCGCAATACGTTGACAGACT2680.19707186504989302No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT2640.19413049392974535No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT2620.19265980836967153No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG2480.1823650094491547No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTATAAACAATACC2440.17942363832900707No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT2430.17868829554897014No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC2360.17354089608871176No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2330.17133486774860102No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC2300.16912883940849027No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT2270.16692281106837953No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT2270.16692281106837953No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT2260.1661874682883426No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC2130.15662801214786273No Hit
CAGTAACCCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG2120.1558926693678258No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA2110.1551573265877889No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2100.154421983807752No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCCGGTCTGTGCCAAGTGTTTGCTG2090.15368664102771507No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2030.14927458434749358No Hit
TGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT2030.14927458434749358No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG2020.14853924156745668No Hit
ACATAGAGGTTCCTTGAGCAGGGGTCGTGCAGGTTCTGCATGGTCCCGTG2000.14706855600738283No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC1960.1441271848872352No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC1960.1441271848872352No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA1950.14339184210719827No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1910.1404504709870506No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT1880.13824444264693986No Hit
GGGTAAAGGTTCAGGTATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA1790.13162635762660763No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC1790.13162635762660763No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC1780.13089101484657073No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT1780.13089101484657073No Hit
GCACGGGACCATGCAGAACCTGCACGACCCCTGCTCAAGGAACCTCTATG1750.12868498650646No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC1730.12721430094638617No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1720.12647895816634924No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1720.12647895816634924No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1710.12574361538631232No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1710.12574361538631232No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCGCAAT1710.12574361538631232No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC1710.12574361538631232No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA1700.12500827260627542No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC1650.12133155870609086No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC1630.11986087314601701No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA1620.11912553036598011No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG1620.11912553036598011No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG1600.11765484480590627No Hit
ATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG1590.11691950202586937No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT1580.11618415924583245No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCT1570.11544881646579554No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC1550.1139781309057217No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGGCGAAACGG1540.11324278812568479No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT1540.11324278812568479No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT1540.11324278812568479No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC1530.11250744534564787No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA1530.11250744534564787No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1520.11177210256561096No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA1520.11177210256561096No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC1510.11103675978557405No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAACCCCTGTTCCGA1500.11030141700553713No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT1480.1088307314454633No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA1470.10809538866542638No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC1470.10809538866542638No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT1470.10809538866542638No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA1470.10809538866542638No Hit
GATACATAGAGGTTCCTTGAGCAGGGGTCGTGCAGGTTCTGCATGGTCCC1470.10809538866542638No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG1450.10662470310535256No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA1430.10515401754527874No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA1420.10441867476524182No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC1420.10441867476524182No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA1420.10441867476524182No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT1400.102947989205168No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT1400.102947989205168No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA1400.102947989205168No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT1380.10147730364509415No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTCAATCTAATCTCCTCC1370.10074196086505725No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC1360.10000661808502033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)