FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378086
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC31630.8365821532667171No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA22540.5961606618600002No Hit
CAATAACATAACCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG16510.43667313785752443No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA15100.3993800352300799No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12530.33140608221409945No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11470.30337013272112695No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA11080.29305501922842947No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10270.2716313219743656No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10190.26951540125791484No Hit
CTCTATGGAAGGCGGGCATATTATATAAGAGAGAAACAACACATAGCGCC10170.26898642107880216No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT10010.26475457964590066No Hit
CTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT9710.2568198769592103No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9440.24967864454118904No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8600.2274614770184561No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA8200.21688187343620233No Hit
CTACTGCGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT8200.21688187343620233No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7950.21026962119729373No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7900.208947170749512No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7640.20207042842104705No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7630.20180593833149071No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7520.19889654734637094No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7360.19466470591346943No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7150.1891104140327862No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG7140.18884592394322985No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA7120.18831694376411717No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7100.18778796358500446No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA7060.1867300032267791No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7040.18620102304766642No Hit
TCCTCAGGCCATGCAATGGAACTCCACAACCTTCCACCAAACTCTGCAAG6950.18382061224165933No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6490.17165406812206746No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6440.17033161767428573No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6350.16795120686827864No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG6190.16371936543537713No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6180.1634548753458208No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6170.16319038525626445No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAAAGGGGTAGGCTGCCT6100.16133895462937003No Hit
CTCTATGACCGTTTCTCTTCCGAAAGTGAGACAAGAAATGTGAAACCACA6010.15895854382336294No Hit
CTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6010.15895854382336294No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5970.15790058346513758No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5870.15525568256957412No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5850.15472670239046143No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5830.15419772221134875No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5730.15155282131578532No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5730.15155282131578532No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5720.15128833122622895No Hit
ATGATGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAGAG5610.14837894024110918No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5600.14811445015155283No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5530.1462630195246584No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC5470.14467607898732032No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5460.14441158889776398No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5440.1438826087186513No Hit
CCTCAAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA5430.14361811862909496No Hit
GTACAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC5320.14070872764397518No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5200.13753484656929904No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5170.13674137630063002No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT5150.1362123961215173No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5110.13515443576329195No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5070.13409647540506658No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5060.13383198531551022No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT5030.1330385150468412No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5000.13224504477817217No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4990.1319805546886158No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4940.1306581042408341No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4900.1296001438826087No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4840.12801320334527067No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4830.1277487132557143No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC4700.12431034209148184No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCCGTCCCGAGAATGTTT4630.12245891146458743No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4570.12087197092724936No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4550.12034299074813667No Hit
CTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4410.11664012949434785No Hit
GTATGGATCGGCAGCGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC4370.11558216913612249No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4340.11478869886745342No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4310.11399522859878439No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT4290.1134662484196717No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG4290.1134662484196717No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4290.1134662484196717No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT4270.11293726824055901No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4250.11240828806144633No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4210.11135032770322095No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA4160.11002787725543924No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4140.10949889707632654No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4120.10896991689721386No Hit
CTAGTAAACTGAACCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4090.10817644662854482No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4080.10791195653898848No Hit
GAGTTATCCGTCCCGAGAATGTTTGCCCCAGACCTGCTGCGAGCAAAACA4060.10738297635987579No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4050.10711848627031945No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4050.10711848627031945No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4050.10711848627031945No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4040.1068539961807631No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACG4010.10606052591209407No Hit
CCCAAAGACAGAAGAAAATTGGTAACAGCGGTACAAAGGGACTCAAGATG4000.10579603582253773No Hit
TCCTAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT3940.10420909528519966No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3910.10341562501653062No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3900.10315113492697428No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3850.10182868447919256No Hit
GGTCCTTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTTTTAGA3840.10156419438963622No Hit
GGCTCAGGGCATACTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTA3790.10024174394185449No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA3790.10024174394185449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)