FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378086
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27930.7387208201308697No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA21870.578439825859725No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA14310.37848531815512876No Hit
CAATAACATAACCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG13380.3538877398263887No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13300.35177181910993793No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA11370.3007252318255635No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT10060.2660770300936824No Hit
CTCTATGGAAGGCGGGCATATTATATAAGAGAGAAACAACACATAGCGCC9800.2592002877652174No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9750.25787783731743574No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA9160.24227292203361142No Hit
CTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT8410.22243616531688556No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8170.2160884031675333No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8130.21503044280930791No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7590.20074797797326535No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7540.19942552752548362No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7370.19492919600302577No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7230.19122633474923695No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7150.1891104140327862No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7120.18831694376411717No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA7080.18725898340589178No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7020.1856720428685537No Hit
CTACTGCGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT6910.18276265188343394No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6900.1824981617938776No Hit
TCCTCAGGCCATGCAATGGAACTCCACAACCTTCCACCAAACTCTGCAAG6600.17456345910718724No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6590.1742989690176309No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6590.1742989690176309No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6430.1700671275847294No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6340.1676867167787223No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6300.16662875642049693No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAAAGGGGTAGGCTGCCT6290.1663642663309406No Hit
CTCTATGACCGTTTCTCTTCCGAAAGTGAGACAAGAAATGTGAAACCACA6280.16609977624138422No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6250.1653063059727152No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6230.16477732579360252No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6160.1629258951667081No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6150.16266140507715177No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6050.16001650418158833No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5850.15472670239046143No Hit
CTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5830.15419772221134875No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5770.15261078167401068No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5720.15128833122622895No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5680.1502303708680036No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5660.1497013906888909No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5540.14652750961421473No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC5460.14441158889776398No Hit
CTCTTATATAATATGCCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5450.14414709880820764No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5400.14282464836042594No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT5190.1372703564797427No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5160.13647688621107368No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5160.13647688621107368No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5150.1362123961215173No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5100.1348899456737356No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5090.13462545558417927No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5040.13330300513639753No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5030.1330385150468412No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5010.1325095348677285No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5000.13224504477817217No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5000.13224504477817217No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC4940.1306581042408341No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT4870.1288066736139397No Hit
CCTCAAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA4860.12854218352438335No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4830.1277487132557143No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4800.12695524298704527No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4770.12616177271837625No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4710.12457483218103818No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4690.1240458520019255No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4620.12219442137503109No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4610.12192993128547475No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4590.12140095110636205No Hit
GTACAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC4590.12140095110636205No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4520.11954952047946764No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4460.11796257994212958No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4440.11743359976301689No Hit
ATGATGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAGAG4440.11743359976301689No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT4430.11716910967346054No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4360.11531767904656613No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4330.11452420877789708No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4260.11267277815100267No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4160.11002787725543924No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4120.10896991689721386No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4100.10844093671810116No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4040.1068539961807631No Hit
CTTTTTACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAAA4020.10632501600165041No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4020.10632501600165041No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3970.10500256555386869No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3920.10368011510608698No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACG3910.10341562501653062No Hit
CCTCATCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT3890.10288664483741794No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3860.1020931745687489No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3860.1020931745687489No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGCTTT3830.10129970430007987No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3820.10103521421052353No Hit
CTAGTAAACTGAACCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3800.10050623403141085No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG3790.10024174394185449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)