FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198346
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17810.8979258467526443No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10280.5182862270981012No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA9980.5031611426497131No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC9900.49912778679680964No Hit
GTGCTGGTGCGCAAACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8770.44215663537454747No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8400.4235023645548687No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7340.37006039950389724No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7320.36905206054067136No Hit
TTTCTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT6850.34535609490486324No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6740.3398102306071209No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6620.33376019682776564No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6510.3282143325300233No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6150.31006423119195753No Hit
GTGCTGGTGCGCAAACCAATTTATGCCTACAGCCTCCTATTACATAATCT5760.2904016214090529No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5610.2828390791848588No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5330.2687223336996965No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACTATACGCTGACAGACT5190.26166396095711536No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5070.25561392717776005No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5060.25510975769614713No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5020.25309307976969536No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5000.2520847408064695No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4550.22939711413388725No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4340.2188095550200155No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACTAT4260.214776199167112No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4180.21074284331420848No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4010.20217196212678856No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3990.20116362316356268No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3970.2001552842003368No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT3840.19360108093936856No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA3830.19309691145775565No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3790.19108023353130388No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3620.18250935234388393No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3550.17898016597259336No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT3520.1774676575277545No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3520.1774676575277545No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3440.17343430167485102No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3430.17293013219323808No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3360.1694009458219475No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT3350.16889677634033456No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3330.16788843737710868No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT3320.16738426789549574No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3320.16738426789549574No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3320.16738426789549574No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3280.165367589969044No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3220.16234257307936636No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3210.16183840359775342No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3170.15982172567130165No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3150.1588133867080758No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3150.1588133867080758No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3130.1578050477448499No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG3040.15326752241033348No Hit
ACTATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAATAGGAAGTTT2930.14772165811259114No Hit
GGCCTATTGATTGGAAAGTCTGTCAGCGTATAGTGGGTCTTTTGGGTTTT2930.14772165811259114No Hit
CCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC2930.14772165811259114No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG2930.14772165811259114No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT2920.1472174886309782No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT2860.14419247174130057No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC2860.14419247174130057No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2810.14167162433323585No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG2810.14167162433323585No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT2810.14167162433323585No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT2800.14116745485162294No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAGCGTATAGTGGGTCT2790.14066328537001No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA2780.14015911588839705No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG2770.1396549464067841No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2770.1396549464067841No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2750.13864660744355822No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC2740.13814243796194528No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGTTCATTGCCAC2740.13814243796194528No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2740.13814243796194528No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA2720.13713409899871942No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT2700.13612576003549354No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2670.1346132515906547No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2670.1346132515906547No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA2670.1346132515906547No Hit
CTCCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA2640.1331007431458159No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2640.1331007431458159No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG2610.13158823470097708No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTCGGCCCACT2610.13158823470097708No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC2600.13108406521936414No Hit
CCTATTACATAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACA2580.13007572625613825No Hit
GTATGATGTGATCTTGTGGCAATGAACCATAACATCCAATGACATAGCCC2530.12755487884807357No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2530.12755487884807357No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA2510.12654653988484768No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2480.12503403144000888No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG2480.12503403144000888No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2470.12452986195839594No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG2460.124025692476783No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2430.12251318403194418No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAA2430.12251318403194418No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2430.12251318403194418No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT2420.12200901455033124No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2410.1215048450687183No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG2350.11847982817904067No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC2340.11797565869742772No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC2330.11747148921581478No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT2330.11747148921581478No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC2320.11696731973420185No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA2320.11696731973420185No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT2310.11646315025258891No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG2290.11545481128936302No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA2280.1149506418077501No Hit
GCGTATAGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACAATGTGGTT2280.1149506418077501No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT2230.1124297943996854No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA2220.11192562491807245No Hit
GATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTT2210.11142145543645951No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG2200.1109172859548466No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG2190.11041311647323364No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2180.1099089469916207No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA2180.1099089469916207No Hit
CGGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA2170.10940477751000775No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG2170.10940477751000775No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT2170.10940477751000775No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG2160.10890060802839484No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC2150.10839643854678188No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT2130.107388099583556No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG2130.107388099583556No Hit
AGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC2130.107388099583556No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC2120.10688393010194308No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA2110.10637976062033014No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT2110.10637976062033014No Hit
ATATTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACC2110.10637976062033014No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT2100.10587559113871718No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2100.10587559113871718No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2090.10537142165710424No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT2080.10486725217549132No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG2070.10436308269387838No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA2070.10436308269387838No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC2060.10385891321226542No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT2050.10335474373065248No Hit
GCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCT2050.10335474373065248No Hit
ACATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCG2050.10335474373065248No Hit
GTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGTCATGG2050.10335474373065248No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC2040.10285057424903957No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCGGCTGCGAGCAAAACA2040.10285057424903957No Hit
CTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCCTC2030.10234640476742662No Hit
GAATCCTGCGGACGACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCC2030.10234640476742662No Hit
ATGTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA2020.10184223528581368No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC2010.10133806580420072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)