FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262102
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAAACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19090.7283424010499728No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16370.6245660086531198No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14580.5562719857154848No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAGACAACACGTAGCGCC13220.5043837895170582No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10960.4181578164226141No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10550.4025150513922061No Hit
ATATAAGAGAGAGACAACACGTAGCGCCTCATTCTGTGGGTCACCATATT10400.39679208857620313No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC9640.3677957436417883No Hit
GGCCTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC8860.3380363369985731No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8700.33193184332816994No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8100.3090399920641582No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8090.30865846120975804No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG7960.3036985601025555No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7170.27355762260494004No Hit
CTACAAACCTTGCCCACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6840.2609671044097336No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6540.24952117877772775No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6500.24799505536012698No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6030.23006310520331777No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5940.22662932751371603No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5660.21594646359051056No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5510.21022350077450763No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5490.20946043906570727No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5360.2045005379585047No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAACATAACCGAAAGCCAAA5300.2022113528321035No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5180.19763298257930118No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5170.197251451724901No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5170.197251451724901No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4900.18695011865609573No Hit
TTATATAAGAGAGAGACAACACGTAGCGCCTCATTCTGTGGGTCACCATA4870.18580552609289513No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4860.18542399523849493No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4820.18389787182089415No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4710.179701032422492No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4680.17855643985929143No Hit
CCCTTATCCAATGGCAGATATTTGGTAACATTTGGATAAAACCTAGCAGG4660.17779337815049104No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4650.17741184729609083No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4600.17550419302408984No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4510.1720704153344881No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4480.1709258227712875No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG4430.16901816849928655No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4410.16825510679048614No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4370.16672898337288536No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG4310.1644397982464842No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4280.1632952056832836No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4200.16024295884808207No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4170.15909836628488144No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4160.15871683543048126No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4160.15871683543048126No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4140.15795377372168087No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4090.15604611944967992No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4040.15413846517767893No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3970.15146774919687755No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG3970.15146774919687755No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGCAAACCCTGTTCCGA3930.14994162577927678No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3890.14841550236167597No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3820.14574478638087462No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT3810.14536325552647442No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3670.1400218235648717No Hit
GTATGCATGTATTCAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACA3630.13849570014727092No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT3630.13849570014727092No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3620.1381141692928707No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3610.13773263843847053No Hit
CCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3570.13620651502086975No Hit
GGATAATAGGGTTTAATACCCTTATCCAATGGCAGATATTTGGTAACATT3570.13620651502086975No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3520.13429886074886876No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3520.13429886074886876No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT3490.13315426818566817No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3470.13239120647686778No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3450.1316281447680674No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA3440.1312466139136672No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT3430.130865083059267No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3430.130865083059267No Hit
GTTTAATACCCTTATCCAATGGCAGATATTTGGTAACATTTGGATAAAAC3420.1304835522048668No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCAG3420.1304835522048668No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3380.12895742878726604No Hit
GTATGGATCGGCAGTGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC3310.12628671280646464No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCCTTCACTTT3280.12514212024326407No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3230.12323446597126309No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGCAGATGAGAAGGCACAG3230.12323446597126309No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3210.12247140426246271No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG3170.12094528084486192No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCCACCCGAATGCTCCAG3130.11941915742726116No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA3100.11827456486406057No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3060.11674844144645977No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3050.11636691059205959No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3030.11560384888325918No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3030.11560384888325918No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3030.11560384888325918No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTC2970.11331466375685802No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG2960.11293313290245781No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT2960.11293313290245781No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC2960.11293313290245781No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAGGCCTGCTT2930.11178854033925723No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC2920.11140700948485704No Hit
CCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGT2910.11102547863045685No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAGACAA2910.11102547863045685No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC2900.11064394777605664No Hit
GGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT2890.11026241692165646No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGT2860.10911782435845588No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA2850.10873629350405566No Hit
GACGAAAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCAGGATTCAGC2840.10835476264965548No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT2830.10797323179525528No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG2830.10797323179525528No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT2810.10721017008645489No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT2760.1053025158144539No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2750.10492098496005373No Hit
GCCTACCCCTCTGTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGC2750.10492098496005373No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT2740.10453945410565353No Hit
CTTCTAGATACCGCCTCAGCACTGTATCGGGAAGCCTTAGAGTCTCCTGA2720.10377639239685314No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2720.10377639239685314No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT2710.10339486154245293No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC2700.10301333068805274No Hit
CTCTCTCGTCCCCTTCTCCGTCTTCCGTTTCGACCGACCACGGGGCGCAC2700.10301333068805274No Hit
CATGTATTCAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC2690.10263179983365256No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC2680.10225026897925235No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2660.10148720727045198No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA2660.10148720727045198No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC2650.10110567641605175No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA2650.10110567641605175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)