FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262102
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15470.5902282317571022No Hit
GTGCTGGTGCGCAAACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13680.5219342088194672No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13510.515448184294664No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAGACAACACGTAGCGCC12360.47157213603864145No Hit
ATATAAGAGAGAGACAACACGTAGCGCCTCATTCTGTGGGTCACCATATT10640.4059488290818079No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10390.39641055772180295No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9280.3540606328833813No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8770.3346025593089713No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC7910.3017909058305545No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7720.2945418195969508No Hit
GGCCTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7530.2872927333633471No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7340.2800436471297434No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7250.27660986944014165No Hit
CTACAAACCTTGCCCACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6660.2540995490305301No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6560.25028424048652814No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6560.25028424048652814No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6410.2445612776705252No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5630.21480187102730997No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5580.21289421675530898No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAACATAACCGAAAGCCAAA5370.2048820688129049No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5360.2045005379585047No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5240.19992216770570234No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5130.19572532830730022No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5050.19267308147209863No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4920.18771318036489612No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4920.18771318036489612No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4880.18618705694729532No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4850.18504246438409475No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4710.179701032422492No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4680.17855643985929143No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4650.17741184729609083No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4590.17512266216968966No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4540.17321500789768868No Hit
TTATATAAGAGAGAGACAACACGTAGCGCCTCATTCTGTGGGTCACCATA4540.17321500789768868No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4420.16863663764488634No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4390.16749204508168575No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4360.16634745251848515No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4340.1655843908096848No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4310.1644397982464842No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4250.16215061312008303No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4240.16176908226568282No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4230.16138755141128264No Hit
CCCTTATCCAATGGCAGATATTTGGTAACATTTGGATAAAACCTAGCAGG4230.16138755141128264No Hit
GTATGCATGTATTCAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACA4220.16100602055688243No Hit
CTCTCTCGTCCCCTTCTCCGTCTTCCGTTTCGACCGACCACGGGGCGCAC4170.15909836628488144No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGCAAACCCTGTTCCGA4120.15719071201288048No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4090.15604611944967992No Hit
AGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAA4090.15604611944967992No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA4020.15337540346887854No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4000.15261234176007812No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3950.15070468748807714No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3930.14994162577927678No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG3920.14956009492487657No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3910.1491785640704764No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3730.14231100869127286No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC3720.14192947783687265No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3710.14154794698247247No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA3700.14116641612807226No Hit
GTTTAATACCCTTATCCAATGGCAGATATTTGGTAACATTTGGATAAAAC3660.1396402927104715No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG3640.1388772310016711No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCAG3620.1381141692928707No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3620.1381141692928707No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3590.1369695767296701No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3590.1369695767296701No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT3530.13468039160326895No Hit
GGATAATAGGGTTTAATACCCTTATCCAATGGCAGATATTTGGTAACATT3520.13429886074886876No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3500.13353579904006838No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3420.1304835522048668No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG3390.12933895964166622No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3370.12857589793286583No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3340.12743130536966524No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3310.12628671280646464No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3300.12590518195206446No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCCTTCACTTT3270.12476058938886388No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3220.1228529351168629No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA3180.12132681169926211No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT3150.12018221913606153No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3120.11903762657286095No Hit
GAACAAATGGCACTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCAC3120.11903762657286095No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3110.11865609571846075No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3090.11789303400966036No Hit
GACGAAAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCAGGATTCAGC3090.11789303400966036No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC3080.11751150315526017No Hit
GTATGGATCGGCAGTGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC3070.11712997230085996No Hit
CCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACA3070.11712997230085996No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3060.11674844144645977No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3050.11636691059205959No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3050.11636691059205959No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3010.1148407871744588No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG3010.1148407871744588No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3010.1148407871744588No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC3000.1144592563200586No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT2980.11369619461125821No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCT2930.11178854033925723No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCCACCCGAATGCTCCAG2930.11178854033925723No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2920.11140700948485704No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAGGCCTGCTT2900.11064394777605664No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT2890.11026241692165646No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT2890.11026241692165646No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG2820.10759170094085507No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAGACAA2810.10721017008645489No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA2810.10721017008645489No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA2800.1068286392320547No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2800.1068286392320547No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC2800.1068286392320547No Hit
GGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT2790.10644710837765449No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT2790.10644710837765449No Hit
ATATTATATAAGAGAGAGACAACACGTAGCGCCTCATTCTGTGGGTCACC2780.10606557752325431No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2780.10606557752325431No Hit
CATGTATTCAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC2760.1053025158144539No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG2760.1053025158144539No Hit
CTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGT2760.1053025158144539No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA2750.10492098496005373No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG2720.10377639239685314No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT2680.10225026897925235No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT2680.10225026897925235No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2680.10225026897925235No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA2670.10186873812485216No Hit
CTTCTAGATACCGCCTCAGCACTGTATCGGGAAGCCTTAGAGTCTCCTGA2670.10186873812485216No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC2670.10186873812485216No Hit
CCCGAATGCTCCAGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGT2660.10148720727045198No Hit
ATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA2660.10148720727045198No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2650.10110567641605175No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC2650.10110567641605175No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA2640.10072414556165157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)