FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348825
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16240.4655629613703146No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14510.41596789220956065No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12870.36895291335196734No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9620.27578298573783416No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8600.2465419623020139No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8420.2413817816956927No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7940.22762130007883608No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7920.22704794667813372No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACAAAACTCTGCAAG7790.2233211495735684No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7440.21328746506127713No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC7420.2127141116605748No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7120.20411381065003942No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA7020.20124704364652765No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6960.19952698344442055No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6950.1992403067440694No Hit
AATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT6870.19694689314125996No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6820.19551350963950406No Hit
CATCTAAACCCTAACAAAACCAAAAGATGGGGTTACTCTTTACATTTCAT6700.1920733892352899No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6680.19150003583458752No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6620.18977997563248047No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6480.18576650182756396No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT6480.18576650182756396No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6450.18490647172651042No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6440.18461979502615924No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6350.18203970472299866No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6340.18175302802264745No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6300.18060632122124273No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6220.17831290761843333No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6210.17802623091808212No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6000.17200602021070738No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5950.17057263670895148No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5920.16971260660789794No Hit
GTTTTGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGCGTGTT5920.16971260660789794No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5910.16942592990754676No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5900.16913925320719558No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC5850.1677058697054397No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGT5840.1674191930050885No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5810.16655916290403497No Hit
CCACCAAACTCTGCAAGATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTG5770.16541245610263025No Hit
GGCAAGCAATTCTGTGCTGGGGGGACCTAATGACTCTAGCTACCTGGGTG5700.163405719200172No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5520.1582455385938508No Hit
GAATTGCTTGCCTGAGTGCAGTATGGTGAGGTGAACAATGCTCAGGAGAC5390.15451874148928546No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5350.15337203468788074No Hit
GCCTGAGGATGCGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5320.1525120045868272No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5220.1496452375833154No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5180.14849853078191072No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5140.14735182398050597No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTT5140.14735182398050597No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCGCGCATGCGCTGATGGCC5130.1470651472801548No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5080.14563176377839893No Hit
CCACAAAACTCTGCAAGATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTG5070.14534508707804772No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5010.14362502687594067No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4980.14276499677488713No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTACG4940.1416182899734824No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACACTCTTTGATTTTCTGT4940.1416182899734824No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4870.13961155307102416No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4720.13531140256575647No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATCTAAACCCTAACAAA4680.13416469576435175No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4600.1318712821615423No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4520.12957786855873288No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4490.12871783845767934No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4470.12814448505697698No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4470.12814448505697698No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4440.12728445495592347No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4330.12413101125206048No Hit
CTTCTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4270.1224109510499534No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4270.1224109510499534No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4240.12155092094889988No Hit
CCATATTCGTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA4200.12040421414749515No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4180.1198308607467928No Hit
CTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4180.1198308607467928No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4160.11925750734609045No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTC4110.11782412384433456No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4100.11753744714398337No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4080.11696409374328103No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4080.11696409374328103No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4000.11467068014047158No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3920.11237726653766215No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3920.11237726653766215No Hit
GTCCCGTGCTGGTAGTTGAAGTTCCTGGAATTAGAGGACAAACGGGCAAC3900.11180391313695978No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3900.11180391313695978No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3880.11123055973625744No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3830.10979717623450154No Hit
TGGTTACAGCGGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCC3820.10951049953415036No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3810.1092238228337992No Hit
GTACAGAAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA3770.10807711603239448No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3770.10807711603239448No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGAACTTCAACTACC3770.10807711603239448No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG3760.10779043933204328No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3750.10750376263169212No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3750.10750376263169212No Hit
CACTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCTT3740.10721708593134094No Hit
CATGTATTTCTGTGCTGGGGGGACCTAATGACTCTAGCTACCTGGGTGGG3730.10693040923098975No Hit
CTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCTTGT3710.1063570558302874No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3700.10607037912993621No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3700.10607037912993621No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3670.10521034902888268No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3660.10492367232853149No Hit
CTGCCTCGTCGTCTAACAACAGTAGTCTCCGGAAGTGTTGATAAGATAGG3640.10435031892782914No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC3640.10435031892782914No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3600.10320361212642441No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA3600.10320361212642441No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3580.10263025872572207No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3580.10263025872572207No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3580.10263025872572207No Hit
CCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTCTGTG3570.1023435820253709No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG3560.10205690532501971No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGC3560.10205690532501971No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3550.10177022862466853No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3540.10148355192431736No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3510.10062352182326383No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3500.10033684512291263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)