FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372895
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23850.6395902331755589No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21210.5687928237171321No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11930.3199292025905416No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11570.3102750103916652No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11300.30303436624250796No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10880.29177114201048554No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10740.28801673393314475No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC10650.2856031858834256No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT9790.26254039340833213No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9070.2432320090105794No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC9020.2418911489829577No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8800.23599136486142214No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8540.2290188927177892No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8500.22794620469569182No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8440.22633717266254574No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG8150.21856018450233983No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7750.20783330428136604No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7680.20595610024269567No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7670.20568792823717133No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7530.20193352015983052No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7490.20086083213773312No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7370.19764276807144104No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA7350.19710642406039233No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7340.196838252054868No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC7170.19227932796095415No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT6990.187452231861516No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGCATT6900.1850386838117969No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6660.17860255567921263No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6640.17806621166816394No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6620.17752986765711526No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6550.17565266361844487No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6530.17511631960739618No Hit
AGCTGGAGGAGTGCGGATCCACACTCCGAAAGACACCAAATATTCTATGA6490.1740436315852988No Hit
GCTTTAATGCCTTTGTATGCATGCATTCAATCTAAGCAGGCTTTCACTTT6410.17189825554110408No Hit
CCTCTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6310.16921653548586063No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6100.16358492336984942No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6090.16331675136432508No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6090.16331675136432508No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6060.16251223534775205No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6030.16170771933117903No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5950.15956234328698427No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5950.15956234328698427No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5860.1571487952372652No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5810.15580793520964348No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5690.15258987114335135No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT5670.15205352713230263No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5650.15151718312125398No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCG5510.14776277504391316No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT5460.14642191501629145No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5450.1461537430107671No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5370.14400836696657238No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5320.14266750693895064No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5250.14079030290028025No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5230.14025395888923156No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5210.13971761487818285No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5150.1381085828450368No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5150.1381085828450368No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5110.13703589482293943No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5080.1362313788063664No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5060.13569503479531772No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5040.13515869078426904No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4990.13381783075664733No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4950.13274514273454993No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4940.13247697072902562No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4880.13086793869587954No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4850.1300634226793065No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4780.1281862186406361No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4740.12711353061853872No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4740.12711353061853872No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4560.12228643451910055No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4550.12201826251357621No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4540.12175009050805186No Hit
GTCATAGAATATTTGGTGTCTTTCGGAGTGTGGATCCGCACTCCTCCAGC4530.12148191850252753No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4520.12121374649700317No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4470.11987288646938146No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4430.11880019844728408No Hit
TTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4430.11880019844728408No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC4420.11853202644175975No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4380.11745933841966237No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4350.11665482240308933No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4320.1158503063865163No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4300.11531396237546762No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4290.1150457903699433No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAAGAGGGA4270.1145094463588946No Hit
ATCCCAGAGTGCGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4270.1145094463588946No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4260.11424127435337025No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4260.11424127435337025No Hit
CCCCAAAAGGCCTCCGTGTGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4240.11370493034232156No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGCATTCAATCTAAGC4220.11316858633127287No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4190.11236407031469985No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4180.11209589830917549No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4170.11182772630365116No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4170.11182772630365116No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4150.11129138229260248No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4140.11102321028707814No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4130.11075503828155378No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4130.11075503828155378No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4130.11075503828155378No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4090.10968235025945641No Hit
CTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCACCCACCCAGGTG4080.10941417825393207No Hit
ATCCAATATCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGC4060.10887783424288339No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4040.1083414902318347No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA4010.10753697421526168No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3950.10592794218211561No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3950.10592794218211561No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3930.10539159817106693No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3910.10485525416001823No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3900.1045870821544939No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3890.10431891014896955No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA3890.10431891014896955No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3890.10431891014896955No Hit
ACCTAGTAGTTAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA3870.10378256613792086No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC3870.10378256613792086No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG3870.10378256613792086No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3800.10190536209925047No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3790.10163719009372611No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3780.10136901808820177No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3770.10110084608267744No Hit
AAACAACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAA3760.10083267407715309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)