FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483675
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG19530.40378353233059383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13480.2786995399803587No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT13170.27229027756241275No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12090.24996123429989145No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC10620.219568925414793No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10170.2102651573887424No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9950.20571664857600663No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9020.18648886132216883No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7650.15816405644285936No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7190.1486535380162299No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC7110.1469995348115987No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6690.13831601798728485No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6080.12570424355197188No Hit
TTCTTCTACTGTGCCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6060.1252907427508141No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT6040.12487724194965627No Hit
GTCCTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5990.12384348994676177No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5970.12342998914560398No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5810.12012198273634156No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5730.11846797953171036No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5730.11846797953171036No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5620.11619372512534243No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG5600.11578022432418462No Hit
GTCCAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5470.11309246911665892No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5350.1106114643097121No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5260.10875071070450198No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5060.10461570269292397No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4940.10213469788597715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)