FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584274
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT28330.48487524688759037No Hit
CTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT25630.4386640514553104No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19760.33819748953401996No Hit
ATTGATAGGTGTCAAAAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG17300.2960939559179426No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC17030.2914728363747146No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG15400.26357496653967144No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT15360.262890356236971No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC14930.2555307954829412No Hit
ATATAAGAGAGAGACCACACGTAGCGCATCATTTTGCGGGTCACCATATT14580.24954045533431232No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14310.2449193357910843No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT14240.24372126776135852No Hit
ATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG13350.22848868852627363No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC13150.22506563701277144No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAT12760.21839068656144206No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG12670.21685031338036603No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC12610.21582339792631539No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12580.21530994019929006No Hit
GTATAATATGATCCTGTGGCAATGATCCCCAACTTCCAATGACGTAACCC12440.21291380413983851No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12270.21000421035336161No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATATTAGGAAAGGAAGGAGTTT11990.20521193823445852No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC11680.19990620838853004No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA11640.1992215980858296No Hit
CTATATAAGAGAGAGACCACACGTAGCGCATCATTTTGCGGGTCACCATA11390.1949427836939518No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC10980.18792552809127225No Hit
ATATGATCCTGTGGCAATGATCCCCAACTTCCAATGACGTAACCCATGAA10290.17611600036968955No Hit
GGATCATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9510.16276609946703088No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9290.15900074280217844No Hit
TTATTGATAGGTGTCAAAAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9260.1584872850751531No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9230.15797382734812776No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9130.15626230159137666No Hit
GTATCCCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTGCCT8890.15215463977517396No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT8870.15181233462382376No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8870.15181233462382376No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT8470.1449662315968193No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA8400.14376816356709352No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAATATTCATTTACAAG8330.14257009553736774No Hit
GGGTATACATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7990.13675090796441397No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7810.13367016160226194No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT7790.13332785645091172No Hit
CTCTCTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTG7740.13247209357253617No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7730.13230094099686107No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA7710.13195863584551085No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTGA7700.13178748326983572No Hit
ATATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCT7680.1314451781184855No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7670.1312740255428104No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT7580.1297336523617344No Hit
CTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATT7210.12340100706175526No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTATATTC7180.12288754933472994No Hit
GGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAATGGAGCAG7100.12151832872932904No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG6940.11877988751852726No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6800.1163837514590757No Hit
GTATACATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC6620.1133030050969237No Hit
GCTAAAGTCATCACATCTCCCCAGCAGAGAATGGCTTGCCTGATTGCAGT6550.11210493706719793No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6430.11005110615909658No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGGTCACCCGTG6410.10970880100774635No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTATATTCCTCTACAG6390.10936649585639613No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6260.10714151237261968No Hit
GCCTAGTCATCTCTTGTTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG6250.10697035979694458No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGACGATGGGATGGGAATACAGGTG6240.10679920722126947No Hit
CTATCAATAATGTCCTCTTGTAAATGAATATTAGGAAAGGAAGGAGTTTG6210.10628574949424413No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6190.1059434443428939No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTATATTCCTCTACAGTACCTATC6190.1059434443428939No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT6180.1057722917672188No Hit
TTCTCGGGGGGTCACCCGTGTGTCTTGGCCAAAATTCGCAGTGCCCAACC6160.10542998661586858No Hit
GTACAACATCGTGAAGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6140.10508768146451836No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6140.10508768146451836No Hit
GGGCTATATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCC6100.10440307116181792No Hit
GTCTAGGAGATCTCTGGCGGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA6090.10423191858614281No Hit
GCCTAATCATCTCTTGTTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG6080.1040607660104677No Hit
CCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGCGGTATT6010.10286269798074192No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5960.10200693510236637No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5900.1009800196483157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)