FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584274
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT29100.498053995214574No Hit
CTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT25380.43438523706343257No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19730.33768403180699463No Hit
ATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG15650.26785378093154927No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT15620.2673403232045239No Hit
ATATAAGAGAGAGACCACACGTAGCGCATCATTTTGCGGGTCACCATATT15490.26511533972074747No Hit
ATTGATAGGTGTCAAAAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15360.262890356236971No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT15320.26220574593427054No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC15260.2611788304802199No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG15230.26066537275319457No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC14250.24389242033703365No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14160.24235204715595762No Hit
GTATAATATGATCCTGTGGCAATGATCCCCAACTTCCAATGACGTAACCC14000.23961360594515588No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG13520.23139828231275053No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC13380.22900214625329895No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC13110.22438102671007099No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAT12990.22232719580196963No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATATTAGGAAAGGAAGGAGTTT12850.2199310597425181No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA12580.21530994019929006No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11720.2005908186912305No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC11490.19665430945070292No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC11370.19460047854260157No Hit
GGATCATATTATACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA11330.19391586823990115No Hit
CTATATAAGAGAGAGACCACACGTAGCGCATCATTTTGCGGGTCACCATA10700.18313325597236912No Hit
ATATGATCCTGTGGCAATGATCCCCAACTTCCAATGACGTAACCCATGAA10680.1827909508210189No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10300.17628715294536468No Hit
GTATACAGGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9350.1600276582562291No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9120.15609114901570154No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT9040.15472192841030064No Hit
TTATTGATAGGTGTCAAAAATTTGTGGGCCCTCTCACTGTAAATGAAAAG8830.1511277243211233No Hit
GTATCCCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTGCCT8740.1495873511400473No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8580.1468489099292455No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTGA8570.14667775735357041No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8530.14599314705086996No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAATATTCATTTACAAG8510.14565084189951974No Hit
GCCTAGTCATCTCTTGTTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG8310.14222779038601752No Hit
ATATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCT8300.14205663781034242No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA8280.1417143326589922No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT8220.14068741720494152No Hit
GGGTATACATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8130.13914704402386552No Hit
CTCTCTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTG7920.13555283993468817No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7890.13503938220766284No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT7630.13058941524010995No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7580.1297336523617344No Hit
GCCTAATCATCTCTTGTTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG7460.12767982145363305No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG7400.12665290599958237No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA7240.12391446478878061No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7160.12254524418337971No Hit
CTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATT7090.12134717615365394No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7080.12117602357797881No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTATATTC7050.12066256585095349No Hit
GTATACATTTGAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC7000.11980680297257794No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT6950.11895104009420236No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6860.11741066691312638No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGGTCACCCGTG6820.11672605661042593No Hit
TTCTCGGGGGGTCACCCGTGTGTCTTGGCCAAAATTCGCAGTGCCCAACC6550.11210493706719793No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6460.11056456388612193No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6460.11056456388612193No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAATATTCA6430.11005110615909658No Hit
GCTATATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT6310.10799727525099526No Hit
GGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAATGGAGCAG6290.10765497009964503No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT6260.10714151237261968No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAAT6160.10542998661586858No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA6110.10457422373749302No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCCGCCAG6080.1040607660104677No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6080.1040607660104677No Hit
GGGCTATATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCC6060.10371846085911747No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG6050.10354730828344236No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5990.1025203928293917No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCCTGTAT5940.10166462995101615No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5890.10080886707264057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)