FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363662
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11880.3266769692736662No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11210.3082532681445958No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9430.25930671887631923No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9230.25380710659898476No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9180.2524322035296512No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8480.23318356055898057No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7660.21063515022190935No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7370.20266071241977443No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7240.19908596443950702No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7060.194136313389906No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6160.169388058141901No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6030.1658133101616336No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5940.1633384846368331No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5670.15591400806243158No Hit
GTTAATATGGGCGTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG5630.15481408560696472No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTG5630.15481408560696472No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5580.15343918253763109No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5440.14958945394349699No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5140.1413400355274953No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5100.1402401130720284No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5010.13776528754722792No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAATTGCCTGTAAATAGACCTAT4970.13666536509176103No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4900.13474050079469396No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4900.13474050079469396No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4870.13391555895309382No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT4830.1328156364976269No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4790.13171571404216004No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4780.1314407334282933No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT4660.12814096606189262No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA4610.12676606299255902No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG4510.1240162568538918No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4500.12374127624002508No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4450.12236637317069148No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC4330.11906660580429079No Hit
CATATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTTGATCTT4290.11796668334882392No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT4180.11494189659628996No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC4140.11384197414082307No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4100.11274205168535617No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4070.11191710984375601No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4050.11136714861602257No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4040.11109216800215585No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3980.10944228431895552No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3980.10944228431895552No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA3940.10834236186348861No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3870.10641749756642156No Hit
GCTAGATACCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTT3800.10449263326935451No Hit
CAAGCACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT3770.10366769142775435No Hit
GTATAAAGAATTTGGAGCTTCTGCCGAGTTACTCTCTTTTTTGCCTTCTG3750.10311773020002091No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC3750.10311773020002091No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCACGTGTTACA3680.10119286590295384No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA3670.10091788528908711No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC3650.10036792406135368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)