FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534004
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA48710.9121654519441802No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC26500.4962509644122516No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT20590.38557763612257584No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGT18830.3526190815050074No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18640.34906105572242907No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15390.28820008838885103No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15180.2842675335765275No Hit
TTTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTT14070.2634811724256747No Hit
ACTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT13170.24662736608714542No Hit
GCCTATTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATG12970.24288207578969448No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC12900.2415712241855866No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC11910.2230320372132044No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11880.22247024366858675No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG11370.21291975341008684No Hit
CTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC11240.2104853147167437No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAACACCGTG10190.1908225406551262No Hit
AGAATGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10090.18894989550640068No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGA9670.1810847858817537No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGT9540.17865034718841058No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9500.1779012891289204No Hit
CTACAAACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC8790.1646055085729695No Hit
CTCTAAAACTGTTTCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8790.1646055085729695No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG8490.15898757312679307No Hit
CATTTACACCAAGACATTATCACAAAATGTGAACAGTTTGTAGGCCCCCT8470.15861304409704796No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT8450.15823851506730288No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT8160.152807844135999No Hit
GTACATTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8120.1520587860765088No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7970.14924981835342058No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTTTTGTGGTTTCACATTTCTT7700.1441936764518618No Hit
ATCACAAAATGTGAACAGTTTGTAGGCCCCCTCACAGTTAATGAGAAAAG7600.1423210313031363No Hit
CTCCAAAAGACACCAAATACTCTAAAACTGTTTCTTTTCCAAAAGTGAGA7540.141197444213901No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGAATCTA7380.13820121197594026No Hit
CAGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCG7380.13820121197594026No Hit
GGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT7290.13651583134208733No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT7240.1355795087677246No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7200.1348304507082344No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA6930.1297743088066756No Hit
GTTAATGAGAAAAGAAGATTACAATTGATTATGCCTGCTAGGTTTTATCC6910.1293997797769305No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC6900.12921251526205796No Hit
TCTAGAGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT6870.12865072171744033No Hit
CAGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCG6790.12715260559845995No Hit
GTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCA6720.1258417539943521No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAAAAAGGCCTTGTAAGTTGGCGA6640.12434363787537173No Hit
CGGGAACCTCAATGTTAATATTCCTTGGACTCATAAGGTGGGGAACTTTA6560.12284552175639134No Hit
GTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6560.12284552175639134No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAA6500.12172193466715604No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTT6500.12172193466715604No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6280.11760211533996001No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6270.11741485082508746No Hit
ATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTG6210.11629126373585218No Hit
ATCACAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAG6170.11554220567636198No Hit
CCTACAAACTGTTCACATTTTGTGATAATGTCTTGGTGTAAATGTATATT6120.11460588310199923No Hit
GTTTTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC6120.11460588310199923No Hit
GTTAACAGGCCTATTGATTGGAAAGTATGTCAACGCATTGTGGGTCTTTT6090.1140440895573816No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGACAAGGGGATTAAACCTTAT6030.11292050246814632No Hit
GGATAATAAGGTTTAATCCCCTTGTCCAATGGTAAATATTTGGTAACCTT5960.11160965086403848No Hit
GGTCCGGCATGGTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGAC5960.11160965086403848No Hit
GTGCAGTATGGTGCGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGA5930.11104785731942082No Hit
GTTTGTAGGCCCCCTCACAGTTAATGAGAAAAGAAGATTACAATTGATTA5890.11029879925993064No Hit
ATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAG5860.109737005715313No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC5860.109737005715313No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC5770.10805162508146006No Hit
TTTTGTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTT5730.10730256702196987No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGTAATCTTCTTTTCTCA5730.10730256702196987No Hit
ATCAAAAAATGTGAACAGTTTGTAGGCCCCCTCACAGTTAATGAGAAAAG5610.1050553928434993No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5520.10337001220964637No Hit
ATATTTACCATTGGACAAGGGGATTAAACCTTATTATCCAGAACATCTAG5490.10280821866502873No Hit
AATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTA5450.10205916060553853No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCT5450.10205916060553853No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5440.10187189609066599No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGT5430.10168463157579344No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5430.10168463157579344No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5380.1007483090014307No Hit
GTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA5370.10056104448655816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)