FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534004
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGA43330.8114171429427495No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC24450.4578617388633793No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT21170.3964389779851836No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18650.3492483202373016No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGT15930.2983123721919686No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13960.26142126276207667No Hit
GCCTATTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGTATGATG13950.2612339982472041No Hit
TTTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTT13090.24512924996816504No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12670.23726414034351803No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11740.21984854046037108No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC11450.2144178695290672No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG10750.20130935348798887No Hit
ACTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT10730.20093482445824373No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTC10650.19943670833926339No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAACACCGTG10440.1955041535269399No Hit
CTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC9300.17415599883146943No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGT8870.1661036246919499No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTGGTTTGGA8810.1649800376027146No Hit
CTCTAAAACTGTTTCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8580.160672953760646No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8320.15580407637395974No Hit
AGAATGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8160.152807844135999No Hit
CATTTACACCAAGACATTATCACAAAATGTGAACAGTTTGTAGGCCCCCT7970.14924981835342058No Hit
GTACATTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7880.14756443771956765No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT7760.14531726354109709No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG7600.1423210313031363No Hit
ATCACAAAATGTGAACAGTTTGTAGGCCCCCTCACAGTTAATGAGAAAAG7500.14044838615441083No Hit
GGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT7490.14026112163953827No Hit
CTACAAACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC7350.1376394184313226No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7200.1348304507082344No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7070.1323960120148913No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT6990.1308978958959109No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6980.13071063138103833No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTTTTGTGGTTTCACATTTCTT6970.1305233668661658No Hit
CTCCAAAAGACACCAAATACTCTAAAACTGTTTCTTTTCCAAAAGTGAGA6900.12921251526205796No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA6850.12827619268769522No Hit
CAGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCG6750.12640354753896973No Hit
GTTTTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC6640.12434363787537173No Hit
ATCACAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAG6430.12041108306304821No Hit
GTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCA6390.11966202500355802No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6380.11947476048868547No Hit
GTTAATGAGAAAAGAAGATTACAATTGATTATGCCTGCTAGGTTTTATCC6370.11928749597381293No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGAATCTA6330.11853843791432275No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCAC6330.11853843791432275No Hit
GTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6280.11760211533996001No Hit
GTTTGTAGGCCCCCTCACAGTTAATGAGAAAAGAAGATTACAATTGATTA6240.11685305728046981No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAA6210.11629126373585218No Hit
GTTAACAGGCCTATTGATTGGAAAGTATGTCAACGCATTGTGGGTCTTTT6170.11554220567636198No Hit
CAGTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCG6000.11235870892352866No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT5950.11142238634916592No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGTAATCTTCTTTTCTCA5780.10823888959633263No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC5760.1078643605665875No Hit
GGATAATAAGGTTTAATCCCCTTGTCCAATGGTAAATATTTGGTAACCTT5750.10767709605171497No Hit
TCTAGAGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT5730.10730256702196987No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC5670.10617897993273459No Hit
GCTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG5660.10599171541786205No Hit
GTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACA5600.10486812832862676No Hit
TTTTGTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTT5570.10430633478400911No Hit
GGGTAAAGGTTCAGGTATTGTTTACATAAAAAGGCCTTGTAAGTTGGCGA5440.10187189609066599No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5370.10056104448655816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)