FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444805
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20840.4685199132204No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC13650.3068760468070278No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT12270.27585121570126236No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11150.2506716426299165No Hit
ATTCTATGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAA10220.22976360427603104No Hit
GGCCTACATATTGTTGACATCTATCAATAATGTCCTCCCGTAAATGAATG9750.21919717629073415No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9010.2025606726543092No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG8420.18929643326851092No Hit
ATGTAGGCCCTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATG7570.17018693584829309No Hit
GGCCCACATAGTGTTGACATCTATCAATAATGTCCTCCCGTAAATGAATG7450.16748912444779174No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7160.16096941356324682No Hit
CAATCACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA7070.1589460550128708No Hit
ATATCTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6860.15422488506199344No Hit
CTGCAGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCGAAA6780.1524263441283259No Hit
GGATACTAACATTGGGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA6580.147929991794157No Hit
CTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG6370.14320882184327963No Hit
ATGTAATTGGAAGTTGGGGCACTTTACCACAGGAACATATTGTACAAAAA6290.14141028090961208No Hit
GTTCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6220.13983655759265295No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6150.13826283427569383No Hit
GTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGC6110.13736356380886006No Hit
ATCCTAACGTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6080.13668911095873473No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6000.13489057002506716No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5970.1342161171749418No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG5800.13039421769089826No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAAGCTTACAGACCACCAAATGCC5750.12927012960735604No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAACGTT5740.1290453119906476No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5740.1290453119906476No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5710.12837085914052224No Hit
ACTTCACTGGACTTTATTCTTCTACTGTACCTGTCTTTAATTCTGAGTGG5680.1276964062903969No Hit
GGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAA5620.12634750059014624No Hit
GTCCCGTGCTGGTCGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5610.12612268297343782No Hit
TTGACATACTTTCCAATCAATAGGTCGATTTACAGGCAGTTTCCGAAAAC5560.12499859488989556No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5490.12342487157293645No Hit
CTTTAGGACATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA5480.123200053956228No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5470.12297523633951957No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5240.11780443115522532No Hit
ATATTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5230.11757961353851688No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5060.11375771405447331No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5060.11375771405447331No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5040.11330807882105641No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5010.11263362597093109No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5010.11263362597093109No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4960.11150953788738886No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4920.11061026742055509No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4910.11038544980384662No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4910.11038544980384662No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC4860.1092613617203044No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4840.10881172648688751No Hit
GCGCATCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCAT4800.10791245602005373No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4800.10791245602005373No Hit
GGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA4780.10746282078663684No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4740.10656355031980307No Hit
ATGTAATTGGAAGTTGGGGCACTTTACCACAAGAACATATTGTACAAAAA4730.10633873270309462No Hit
GTTTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4650.10454019176942704No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4640.1043153741527186No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG4610.10364092130259327No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA4580.10296646845246793No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4570.1027416508357595No Hit
CTACTGTACCTGTCTTTAATTCTGAGTGGCAAACTCCCTCCTTTCCTAAC4560.10251683321905104No Hit
GTTCCTGTGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAAGTTA4550.1022920156023426No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTA4490.10094310990209193No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4470.10049347466867503No Hit
CACAAGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCT4470.10049347466867503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)