FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430180
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30360.7057510809428611No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA25470.592077734901669No Hit
CTCTAATACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14600.33939281231112556No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12440.289181272955507No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC9750.22664930959133386No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9450.21967548468083126No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9180.21339904226137896No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8960.20828490399367708No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8640.20084615742247433No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8530.19828908828862335No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8110.18852573341391976No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT7950.1848063601283184No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7620.17713515272676553No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7620.17713515272676553No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7240.16830164117346227No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6840.15900320795945885No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6800.1580733646380585No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6800.1580733646380585No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT6540.15202938304895625No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6460.15016969640615557No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6360.1478450881026547No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6170.14342833232600308No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6080.1413361848528523No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6020.1399414198707518No Hit
GTATTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5960.13854665488865125No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5900.13715188990655075No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5810.13505974243339997No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCGGTTAACAGGAAGTTT5430.1262262308800967No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5210.12111209261239482No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG5070.1178576409874936No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5030.11692779766609328No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5010.11646287600539308No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTTTCCATGC4910.11413826770189223No Hit
CTATTCTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTT4770.11088381607699102No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC4750.11041889441629085No Hit
ATTCTTCTACTGTTCCTGTCTTTAACCCTTATTGGAAAACACCCTCTTTT4640.10786182528243991No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAAT4580.1064670603003394No Hit
CTAATACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT4570.10623459946998931No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4560.10600213863963923No Hit
CTGTAATGTCAACGACCGACCTTGAGGCTTACTTCAAAGACTGTTTGTTT4540.10553721697893904No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4470.10390999116648846No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC4390.10205030452368777No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT4370.10158538286298759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)