FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406833
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA34760.854404632859183No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15470.3802543058208159No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15060.3701764606116023No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13740.33773071505998775No Hit
CCTTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGA11700.28758729011658346No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11520.283162870268636No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10470.25735375448894265No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC10110.2485049147930478No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9580.23547745635186917No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8570.2106515449828308No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8540.20991414167483954No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8330.2047523185189009No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8120.1995904953629622No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT8100.19909889315763468No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7990.1963950810283335No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7910.1944286722070235No Hit
TCTCAAGGACTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGA7270.17869740163654374No Hit
GTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATTTGGGTAAAAC7220.17746839612322501No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7080.17402718068593254No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7000.1720607718646226No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGTCAGTC6970.17132336855663136No Hit
ATATTTGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG6680.1641951365793827No Hit
GGATAATAAGGTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATT6660.16370353437405522No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC6610.16247452886073646No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6500.15977071673143525No Hit
ATATGTGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG6480.15927911452610777No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6460.15878751232078028No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6320.15534629688348783No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6280.15436309247283284No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6270.15411729137016908No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6190.1521508825488591No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCATGTATT6090.14969287152222166No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6080.1494470704195579No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC6070.14920126931689415No Hit
GAAAAAAAGGTGTTTTCCAATGAGGGTTAAAGACAGGAACAGTAGAAGAA6060.14895546821423042No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6060.14895546821423042No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6050.14870966711156666No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6040.1484638660089029No Hit
CCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTG5980.14698905939292045No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5870.14428524726361922No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5840.14354784395562797No Hit
CCCTTATCCAAAGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG5510.13543640756772435No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5400.13273259543842314No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT5380.13224099323309566No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA5350.1315035899251044No Hit
ACCTAGTAGTTAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA5320.13076618661711317No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5280.12978298220645818No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5200.1278165733851482No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4970.12216314802388205No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4920.12093414251056331No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT4880.11995093809990831No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4860.11945933589458083No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4820.11847613148392583No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4770.11724712597060712No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4770.11724712597060712No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4730.11626392155995212No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4700.11552651825196088No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4690.11528071714929713No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4680.11503491604663339No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4660.1145433138413059No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4660.1145433138413059No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4660.1145433138413059No Hit
ATCCAATATCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGC4630.11380591053331465No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT4610.11331430832798715No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4540.11159370060934094No Hit
ACTCTTTACATTTCATGGGTTATGTCATTGGATGCCATGGATCATTGCCA4520.11110209840401344No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4480.11011889399335846No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCTT4470.1098730928906947No Hit
CTCTTTACATTTCATGGGTTATGTCATTGGATGCCATGGATCATTGCCAC4470.1098730928906947No Hit
ACTGTGGAGTTACTCTCCTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4460.10962729178803096No Hit
TTTCATGGGTTATGTCATTGGATGCCATGGATCATTGCCACAAGATCACA4430.10888988848003972No Hit
GTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCC4410.10839828627471222No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4400.1081524851720485No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4400.1081524851720485No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4330.10643187745340225No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4320.10618607635073851No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4280.10520287194008353No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT4250.10446546863209227No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCTTTGG4230.10397386642676479No Hit
CCTTTTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGA4200.10323646311877356No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4200.10323646311877356No Hit
CTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCACCCACCCAGGTG4180.10274486091344606No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4160.10225325870811856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)