FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406833
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26680.6557973419068758No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13200.32445745551614547No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10850.266694196390165No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10780.26497358867151877No Hit
CCTTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGA9650.23719806407051544No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9630.23670646186518793No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9250.22736601996396558No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9190.2258912133479831No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8100.19909889315763468No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7600.1868088380244474No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7170.17623939060990626No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6760.16616154540069267No Hit
GTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATTTGGGTAAAAC6700.16468673878471018No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6700.16468673878471018No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6380.1568211034994703No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6220.15288828585685035No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6170.15165928034353163No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6040.1484638660089029No Hit
TCTCAAGGACTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGA5890.14477684946894673No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCATGTATT5810.14281044064763673No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5800.142564639544973No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGTCAGTC5630.1383860207996893No Hit
ATATGTGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5530.13592800977305183No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT5460.13420740205440562No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC5420.13322419764375062No Hit
ATATTTGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5370.1319951921304319No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT5360.13174939102776814No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5340.13125778882244066No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5080.12486696015318324No Hit
CCCTTATCCAAAGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG5020.12339215353720076No Hit
GGATAATAAGGTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATT4990.12265475022920952No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4920.12093414251056331No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4770.11724712597060712No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4760.11700132486794335No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4710.11577231935462463No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4670.11478911494396964No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4620.1135601094306509No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA4620.1135601094306509No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4580.11257690501999593No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT4570.11233110391733218No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4520.11110209840401344No Hit
CCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTG4450.1093814906853672No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT4340.10667767855606601No Hit
GAAAAAAAGGTGTTTTCCAATGAGGGTTAAAGACAGGAACAGTAGAAGAA4330.10643187745340225No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC4290.10544867304274727No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4260.10471126973475603No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4230.10397386642676479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)