FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406792
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27170.6679089067631615No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT20820.5118094751125882No Hit
ACCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT14890.3660347302798482No Hit
CCTCCACCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA13630.3350606698263486No Hit
CTCTGATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT12080.2969576589510118No Hit
CATTAGGGTAAAACCTAGCTGGCATAATCAATTGCAATCTTCTTTTCTCA12030.29572852956793644No Hit
ACCCTAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11480.28220810635410726No Hit
GCTTCGATTGAATACTTGCATACAAGGGCATTAAGGCAGGATAACCACAT11320.278274892328266No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10360.2546756081732187No Hit
CATTTGGGTAAAACCTAGCTGGCATAATCAATTGCAATCTTCTTTTCTCA10000.24582587661507602No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9900.24336761784892524No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC9470.23279710515447696No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT8680.21337686090188598No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT8600.21141025388896534No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8590.21116442801235027No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA8080.19862730830498143No Hit
CACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7670.1885484473637633No Hit
AATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGT7260.17846958642254518No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA7230.17773210879269996No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTTTACATTTCAT7220.17748628291608487No Hit
CATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACT7170.1762571535330095No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6950.17084898424747783No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACACC6870.1688823772345572No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAAC6850.16839072548132705No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6810.16740742197486677No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT6770.16642411846840646No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCTGG6610.16249090444256525No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6570.16150760093610492No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACA6520.16027847155302954No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6330.15560777989734312No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA6180.15192039174811697No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6180.15192039174811697No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6100.14995378473519635No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6030.14823300359889083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5960.1465122224625853No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5960.1465122224625853No Hit
GAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCAT5890.14479144132627977No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTTGGACGGAAATTGCACC5870.14429978957304962No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5700.14012074967059335No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA5690.13987492379397826No Hit
ATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5670.1393832720407481No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5660.139137446164133No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5540.1361875356447521No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5320.13077936635922044No Hit
GTATAAAGGGACTCAAGGTGCTGTACAGACTTGGCCCCCAATACCACATC5270.12955023697614507No Hit
CAGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA5190.12758362996322445No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5150.12660032645676414No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5090.12512537119707368No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCCTTGTATGCAAGTATT5020.12340459006076815No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5010.12315876418415309No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT4950.12168380892446262No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4940.12143798304784754No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTTTAC4930.12119215717123248No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4920.1209463312946174No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC4830.11873389840508172No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4780.11750476902200634No Hit
ATTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4770.11725894314539127No Hit
GCTCTATATCGGGATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4770.11725894314539127No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4710.1157839878857008No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA4690.11529233613247064No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4660.11455485850262541No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGGTGGCC4600.11307990324293496No Hit
GTTATTGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC4590.11283407736631988No Hit
CCATAAAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT4580.1125882514897048No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4560.11209659973647466No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTTGGACGGAAATTGC4560.11209659973647466No Hit
GCTTAGATTGAATACTTGCATACAAGGGCATTAAGGCAGGATAACCACAT4540.11160494798324451No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4530.11135912210662943No Hit
ACTGCTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATAT4520.11111329623001436No Hit
GCCTTCCGACTTCTTTCCTTCAGTACGAGACCTTCTAGATACCGCCTCAG4460.1096383409703239No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCAGCTAGGTT4370.10742590808078822No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4370.10742590808078822No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4360.10718008220417315No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4350.10693425632755807No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4340.10668843045094299No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4320.10619677869771285No Hit
CTTTTCCTAATATTCATTTACACCAAGACATTGTCAAAAAATGTGAACAA4270.10496764931463745No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4240.10423017168479222No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4220.10373851993156206No Hit
TAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGAC4190.10300104230171685No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4180.10275521642510177No Hit
CTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4160.10226356467187162No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4140.10177191291864146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)