FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96169
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA6520.6779731514313344No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA3370.3504247730557664No Hit
CAAAATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT3320.3452255924466304No Hit
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT2960.3077914920608512No Hit
CTAGTTCCCTAGATGCTGGATCTTCCAAATTAACACCCACCCAGGTAGCT2500.25995903045679997No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC2290.23812247189842883No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG2280.2370826357766016No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC2100.218365585583712No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1970.20484771599995844No Hit
TACATGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATAC1820.1892501741725504No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT1800.187170501928896No Hit
CTTTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1790.1861306658070688No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAGTTTGTAGGTCCACT1750.18197132131976002No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1740.1809314851979328No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT1700.176772140710624No Hit
GTAGATTTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTACGT1670.1736526323451424No Hit
ACGTGATGTTTCTCTTTTATATAATACACCCGCCTTCCATAGAGTGTGTA1650.171572960101488No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC1640.1705331239796608No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG1570.1632542711268704No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA1560.1622144350050432No Hit
GAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCCAAAGGTTA1540.1601347627613888No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT1540.1601347627613888No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT1540.1601347627613888No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTACGT1510.1570152543959072No Hit
GATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC1460.1518160737867712No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT1450.15077623766494402No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGC1430.14869656542128962No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG1430.14869656542128962No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT1380.1434973848121536No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGACCCGTGGTCGGACGAAACGG1370.1424575486903264No Hit
CCAACAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTA1350.140377876446672No Hit
CAAGAGCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT1330.1382982042030176No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA1320.1372583680811904No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC1310.1362185319593632No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTG1300.135178695837536No Hit
ACAAACTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATTAGG1290.1341388597157088No Hit
CCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTA1260.13101935135022721No Hit
AACAATTTGTAGGTCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTA1240.1289396791065728No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT1230.1278998429847456No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC1220.1268600068629184No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT1190.1237404984974368No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA1190.1237404984974368No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAGTTTGTAGG1180.12270066237560961No Hit
CTGTATGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG1160.1206209901319552No Hit
ACGTGTTGTTTCTCTTTTATATAATACACCCGCCTTCCATAGAGTGTGTA1160.1206209901319552No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1150.119581154010128No Hit
GCCATACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT1140.1185413178883008No Hit
ATATTATTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC1130.11750148176647361No Hit
AATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTAG1120.1164616456446464No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC1110.11542180952281922No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1110.11542180952281922No Hit
GCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGG1110.11542180952281922No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT1100.114381973400992No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT1100.114381973400992No Hit
CAATAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT1100.114381973400992No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG1100.114381973400992No Hit
CTCTATGGAAGGCGGGTGTATTATATAAAAGAGAAACATCACGTAGCGCC1050.109182792791856No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG1030.1071031205482016No Hit
ATGATGAGGTGAACAATGCTCAGGAGACTCTAAGGCATCCCGAAACAGAG1030.1071031205482016No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA1030.1071031205482016No Hit
ATCAAGAAGAGCTCGTACTGAAGGAAAGAAGTCAGCAGGCAAAAACGAGA1020.10606328442637442No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTA1020.10606328442637442No Hit
ACATAAGACAAGAAATGTGAAACCACAATATTTGCCTGAACTTTAGGCCC1020.10606328442637442No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT1020.10606328442637442No Hit
TAATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTA1020.10606328442637442No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG1010.1050234483045472No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC1010.1050234483045472No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC1000.10398361218272No Hit
GTTTGTAGGTCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTA1000.10398361218272No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGC1000.10398361218272No Hit
ATTAACACCCACCCAGGTAGCTAGAGTCATTAGTTCCCCCCAGCAAAGGA970.10086410381723841No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG970.10086410381723841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)