FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96169
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA5740.5968659339288128No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA3450.358743462030384No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA2270.2360427996547744No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC2160.22460460231467522No Hit
CATTTAAACCCTACCAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT1910.19860869926899521No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT1780.1850908296852416No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG1720.1788518129542784No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG1710.1778119768324512No Hit
CTTTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1670.1736526323451424No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA1480.1538957460304256No Hit
GATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC1430.14869656542128962No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT1380.1434973848121536No Hit
CTTCTTGATACCGCCCTAGCTCTGTTTCGGGATGCCTTAGAGTCTCCTGA1320.1372583680811904No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT1320.1372583680811904No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA1300.135178695837536No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT1290.1341388597157088No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC1290.1341388597157088No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC1270.13205918747205442No Hit
TCTTGATACCGCCCTAGCTCTGTTTCGGGATGCCTTAGAGTCTCCTGAGC1240.1289396791065728No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1240.1289396791065728No Hit
CAAAATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT1220.1268600068629184No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC1220.1268600068629184No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG1220.1268600068629184No Hit
GTTTGTAGGTCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTA1160.1206209901319552No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGC1070.11126246503551039No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG1050.109182792791856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1040.10814295667002881No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC1030.1071031205482016No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC1030.1071031205482016No Hit
ACGTGATGTTTCTCTTTTATATAATACACCCGCCTTCCATAGAGTGTGTA1030.1071031205482016No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTACGT1010.1050234483045472No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA1000.10398361218272No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT980.1019039399390656No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA970.10086410381723841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)