FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380753
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA27490.7219903717107942No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14160.37189464035739705No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT11360.2983561521511321No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10760.28259790467836104No Hit
AGTACACTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATAAG10140.26631438228983095No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8890.23348470005489122No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT7900.20748359172481898No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7870.20669567935118044No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7190.18883633221537321No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC7070.185684682720819No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTCGTAGGCCCACT6800.17859347135807202No Hit
GCGTCACCAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6610.1736033596583612No Hit
GGAGACTACTATTGTTAGAGGACGAGGCAGGTCCCCTAGAAGAAGAACTC6570.17255280982684312No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6290.16519896100621664No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6290.16519896100621664No Hit
GGGTCTCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTC5800.15232972557012026No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5790.15206708811224073No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5700.1497033509913251No Hit
TTTGCAAGATCCAACATCCAGGGACCTAGTAGTCAGTTATGTCAACACTA5430.1426121396285781No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT5400.14182422725493954No Hit
TTCTATTACTGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5380.14129895233918052No Hit
ATACCGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5280.13867257776038533No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5090.1336824660606745No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5090.1336824660606745No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT5030.1321066413133974No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG4910.12895499181884318No Hit
CTTTCACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4900.12869235436096366No Hit
CCATATACTTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACCA4780.12554070486640945No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCGCCATGT4750.12475279249277091No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4750.12475279249277091No Hit
GTACACTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATAAGC4740.12449015503489139No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4570.1200253182509396No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4560.11976268079306007No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4530.1189747684194215No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTTCACCAAACTCTGCAAG4440.11661103129850586No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4250.11162091959879503No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC4200.11030773230939744No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT4120.1082066326463613No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC4080.10715608281484322No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA3930.10321652094665044No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3930.10321652094665044No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA3910.10269124603089141No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3900.1024286085730119No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTACGTGTTGTTTCTCTCTTAT3830.10059014636785528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)