FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398243
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT20210.5074791019553387No Hit
TTCCTACATACTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGT16080.4037735754300766No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACCAGA15550.39046511803095096No Hit
GTGCTGGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTC12990.3261827577634761No Hit
TGGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCT11350.28500187071712496No Hit
GTACTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10660.26767576580128216No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9270.23277245299980162No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8780.22046840747985524No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACCAGATGTTCTGGAT8740.21946399560067598No Hit
GGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTT8640.21695296590272775No Hit
GCTAGATACAGTGAGGAGTATAGCCATGAGTAGCACAGGGTCCCTATCCT8640.21695296590272775No Hit
GTCTTTATGACCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTT8520.21393973026518984No Hit
GCCAGAAGATGTGTGCAGTAGGAGTGAGAGGTGGGCTTTCCTACATACTT8490.2131864213558054No Hit
ATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCAGAAAGTCTCTCAGCTGA8270.20766215602031926No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC8020.20138458177544868No Hit
GTATTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7920.1988735520775004No Hit
CCTTAAGAGTCTGGGAACACAGCCCAGAAGGCTTCAGCCTCATTTTACCC7890.19812024316811597No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT7700.19334928674201432No Hit
CCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTTAGAATGCCTT7690.1930981837722195No Hit
GGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTT7660.192344874862835No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7630.19159156595345053No Hit
GGACCAAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTTCAGGC7500.18832722734611781No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT7190.18054303528247828No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7180.18029193231268345No Hit
GTATAGCCATGAGTAGCACAGGGTCCCTATCCTATTGTCAGAGACGTGTG7160.17978972637309382No Hit
TTCTAGTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7110.1785342115241197No Hit
TTCTAATACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7080.17778090261473523No Hit
CATTTAAACCCTTACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT7060.17727869667514556No Hit
ATCCTATTGTCAGAGACGTGTGAACCAGAGCAACTCCGTCTTGAATAGGA6820.1712522254000698No Hit
GTCCCTATCCTATTGTCAGAGACGTGTGAACCAGAGCAACTCCGTCTTGA6700.16823898976253193No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6650.1669834749135578No Hit
TGGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCT6490.16296582739684062No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6420.16120810660827686No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6390.16045479769889237No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT6360.1597014887895079No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6330.15894817988012344No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6270.15744156206135448No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT6250.15693935612176485No Hit
CACCTACTGTATGCAAATTACTGTGCTAGATACAGTGAGGAGTATAGCCA6250.15693935612176485No Hit
CCTACATACTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCA6250.15693935612176485No Hit
CTGTTTTATCAGCAAGGTCTTTATGACCTGTATCTTGTGCCGACATTTTA6160.15467942939361143No Hit
CAGTAGGAGTGAGAGGTGGGCTTTCCTACATACTTCAAATCATCTAGAAG6130.15392612048422696No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA6110.15342391454463733No Hit
CTTTATGACCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTTAG6110.15342391454463733No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGAATGCCTTAGAGTCTCCTGA5920.14865295811853566No Hit
GTGCTAGATACAGTGAGGAGTATAGCCATGAGTAGCACAGGGTCCCTATC5830.14639303139038226No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5770.14488641357161333No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5720.14363089872263918No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5690.1428775898132547No Hit
GAGTAGCACAGGGTCCCTATCCTATTGTCAGAGACGTGTGAACCAGAGCA5650.14187317793407542No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5590.14036656011530646No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5550.1393621482361272No Hit
GTGCTGGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTC5540.13911104526633236No Hit
GAGTTATCTGTCCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA5540.13911104526633236No Hit
CTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCAGAAAGTCT5530.13885994229653753No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5440.13660001556838414No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5350.13434008884023071No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5320.13358677993084625No Hit
CTATTTACACACTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAA5260.1320801621120773No Hit
ACTAGTTCCCTAGATGTTGGATCTTCCAAATTAACACCCACCCAGGTAGC5200.13057354429330836No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT5190.13032244132351353No Hit
GTGCATAGCCAGAAGATGTGTGCAGTAGGAGTGAGAGGTGGGCTTTCCTA5180.1300713383537187No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA5160.12956913241412907No Hit
GTCCCGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5140.1290669264745394No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5040.12655589677659118No Hit
GTATCGGAATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5020.12605369083700152No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4850.12178494035048953No Hit
GTACTGGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4800.12052942550151542No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4780.12002721956192576No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4670.1172650868941827No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG4650.11676288095459304No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4620.11600957204520859No Hit
GCCTTAAGAGTCTGGGAACACAGCCCAGAAGGCTTCAGCCTCATTTTACC4600.11550736610561893No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4570.11475405719623445No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4570.11475405719623445No Hit
GTAGGAGTGAGAGGTGGGCTTTCCTACATACTTCAAATCATCTAGAAGAG4500.1129963364076707No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT4490.11274523343787587No Hit
GTAATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4390.11023420373992762No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4370.10973199780033799No Hit
GTGCAGTAGGAGTGAGAGGTGGGCTTTCCTACATACTTCAAATCATCTAG4340.10897868889095351No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4330.10872758592115868No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4330.10872758592115868No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4330.10872758592115868No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG4320.10847648295136388No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4260.10696986513259492No Hit
ATGTAAGACCTTGGGCAAGCATTGGTGGGCGTTCACGGTGGTCTCCATGC4240.10646765919300528No Hit
CTCTTAAGGCATTCTAAGTCACAGAATGAGATAAAATGTCGGCACAAGAT4240.10646765919300528No Hit
GTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTTGGTTTTG4210.10571435028362079No Hit
CCCCAAAAGGCCTCCGTGTGGTGGGGTGAAACCCAGCCCGAATGCTCCAG4200.10546324731382598No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4160.10445883543464668No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA4160.10445883543464668No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGATTTAATGACTCTA4150.10420773246485185No Hit
CAGTACTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA4110.10320332058567257No Hit
CTTGAACACCTACTGTATGCAAATTACTGTGCTAGATACAGTGAGGAGTA4110.10320332058567257No Hit
CCAATAAACAACACTTGAACACCTACTGTATGCAAATTACTGTGCTAGAT4020.10094339385751915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)