FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398243
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT24120.605660363145115No Hit
GTGCTGGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTC15590.3914695299101302No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACCAGA15410.3869496764538234No Hit
TTCCTACATACTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGT15140.3801698962693632No Hit
TGGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCT12090.30358349048194194No Hit
GTACTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10870.2729489281669734No Hit
GTCTTTATGACCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTT9970.25034966088543925No Hit
ATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCAGAAAGTCTCTCAGCTGA9020.22649487875493102No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACCAGATGTTCTGGAT8820.22147281935903457No Hit
GTATTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8800.2209706134194449No Hit
GGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTT8640.21695296590272775No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8510.21368862729539503No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8270.20766215602031926No Hit
GTATAGCCATGAGTAGCACAGGGTCCCTATCCTATTGTCAGAGACGTGTG8070.20264009662442276No Hit
GCCAGAAGATGTGTGCAGTAGGAGTGAGAGGTGGGCTTTCCTACATACTT8040.2018867877150383No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7850.19711583128893664No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC7830.196613625349347No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7760.19485590456078325No Hit
CCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTTAGAATGCCTT7670.19259597783262983No Hit
CCTTAAGAGTCTGGGAACACAGCCCAGAAGGCTTCAGCCTCATTTTACCC7600.19083825704406607No Hit
GCTAGATACAGTGAGGAGTATAGCCATGAGTAGCACAGGGTCCCTATCCT7500.18832722734611781No Hit
GGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTT7380.18531399170857993No Hit
CATTTAAACCCTTACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT7320.183807373889811No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7230.18154744716165758No Hit
GTCCCTATCCTATTGTCAGAGACGTGTGAACCAGAGCAACTCCGTCTTGA7180.18029193231268345No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT7110.1785342115241197No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT6930.17401435806781287No Hit
TTCTAATACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6890.17300994618863358No Hit
TGGTGGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCT6690.1679878867927371No Hit
TTCTAGTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6690.1679878867927371No Hit
CTTTATGACCTGTATCTTGTGCCGACATTTTATCTCATTCTGTGACTTAG6480.1627147244270458No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6460.16221251848745616No Hit
GGACCAAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTTCAGGC6320.1586970769103286No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT6320.1586970769103286No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT6260.15719045909155965No Hit
CACCTACTGTATGCAAATTACTGTGCTAGATACAGTGAGGAGTATAGCCA6220.15618604721238039No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATA6190.15543273830299592No Hit
CAGTAGGAGTGAGAGGTGGGCTTTCCTACATACTTCAAATCATCTAGAAG6170.15493053236340626No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA6130.15392612048422696No Hit
ATCCTATTGTCAGAGACGTGTGAACCAGAGCAACTCCGTCTTGAATAGGA6110.15342391454463733No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6090.15292170860504767No Hit
CTGTTTTATCAGCAAGGTCTTTATGACCTGTATCTTGTGCCGACATTTTA6060.1521683996956632No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6000.15066178187689427No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5920.14865295811853566No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGAATGCCTTAGAGTCTCCTGA5910.14840185514874085No Hit
GTGCTGGTAGACGTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTC5870.14739744326956156No Hit
GTGCTAGATACAGTGAGGAGTATAGCCATGAGTAGCACAGGGTCCCTATC5860.14714634029976673No Hit
CCTACATACTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCA5850.1468952373299719No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5750.14438420763202367No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5690.1428775898132547No Hit
CTTCAAATCATCTAGAAGAGGGAAAGAAAAATCAGAAGGTCAGAAAGTCT5550.1393621482361272No Hit
GAGTTATCTGTCCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA5540.13911104526633236No Hit
GTGCATAGCCAGAAGATGTGTGCAGTAGGAGTGAGAGGTGGGCTTTCCTA5370.13484229477982035No Hit
CTCTTGTCGTCATCTTGGTTTTGGTGGGTTTTGGCCGGCTTCTTGACTGC5360.13459119181002552No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5300.1330845739912566No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5190.13032244132351353No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT5100.12806251459536014No Hit
GTGTAGCAGTGAAGACAACCGAGGTCACTCTTGTCGTCATCTTGGTTTTG5050.12680699974638598No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4910.12329155816925848No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA4870.12228714629007917No Hit
GAGTAGCACAGGGTCCCTATCCTATTGTCAGAGACGTGTGAACCAGAGCA4870.12228714629007917No Hit
GTCCCGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4860.12203604332028437No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT4860.12203604332028437No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC4790.12027832253172059No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG4780.12002721956192576No Hit
CCCCAAAAGGCCTCCGTGTGGTGGGGTGAAACCCAGCCCGAATGCTCCAG4780.12002721956192576No Hit
GTATCGGAATGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4730.11877170471295165No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA4700.11801839580356717No Hit
GTACTGGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4700.11801839580356717No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA4650.11676288095459304No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4640.11651177798479823No Hit
GTAATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4600.11550736610561893No Hit
GCCTTAAGAGTCTGGGAACACAGCCCAGAAGGCTTCAGCCTCATTTTACC4600.11550736610561893No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4490.11274523343787587No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4350.10922979186074834No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4350.10922979186074834No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4340.10897868889095351No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG4340.10897868889095351No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4300.10797427701177423No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT4270.10722096810238974No Hit
GCCCAGAAGGCTTCAGCCTCATTTTACCCAGCTCCTATTCAAGACGGAGT4230.10621655622321045No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT4190.10521214434403116No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4150.10420773246485185No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATT4080.10245001167628809No Hit
GTAGGAGTGAGAGGTGGGCTTTCCTACATACTTCAAATCATCTAGAAGAG4070.10219890870649329No Hit
ACTAGTTCCCTAGATGTTGGATCTTCCAAATTAACACCCACCCAGGTAGC4070.10219890870649329No Hit
GCCTGAGGATGAGTGTCTCTCAGAGGTGGAGACAGTGGGGTAGGCTGCCT4060.10194780573669845No Hit
CAGTACTAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA4040.10144559979710879No Hit
GTATTAAACCTTATTATCCAGAACATCTGGTTAATCATTACTTCCAAACT4030.10119449682731398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)