FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488674
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14810.3030650290377634No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14780.30245112283444586No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACAGT14030.28710346775150714No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13550.2772809684984264No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12720.26029623020664083No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11890.2433114919148553No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11350.23226118025513942No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10640.21773206677662407No Hit
CATTTAAACCCTCACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT10580.21650425436998902No Hit
GTATAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10570.21629961896888314No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT10470.21425326495782465No Hit
GTCTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG10190.20852347372686086No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10080.20627248431469652No Hit
TTCTATGACCGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9650.19747316206714496No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9390.19215264163839288No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9330.19092482923175777No Hit
CTTATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGTTT8670.1774188927587717No Hit
GTCATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8390.17168910152780792No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8270.16923347671453773No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8230.16841493511011432No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8080.16534540409352655No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA8020.16411759168689147No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7980.16329905008246806No Hit
CTCTTATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGT7710.15777389425261013No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7670.15695535264818672No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7650.15654608184597504No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7600.15552290484044579No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7530.15409045703270483No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA7450.15245337382385804No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7330.14999774901058785No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7320.149793113609482No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7310.14958847820837612No Hit
CTTCTAGATACCGCCTCAGCTCTATTTCGGGAAGCCTTAGAGTCTCCTGA7040.1440633223785182No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6880.14078915596082459No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6840.13997061435640118No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6790.13894743735087195No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6690.13690108333981346No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6680.1366964479387076No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6620.1354686355320725No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6550.13403618772433154No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6510.13321764611990816No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG6440.1317851983121672No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6420.13137592750995553No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6320.129329573498897No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6260.1281017610922619No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6120.12523686547678No Hit
ATACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6030.12339514686682737No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5980.12237196986129813No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5970.12216733446019226No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5950.12175806365798059No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5940.12155342825687473No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA5880.12032561585023963No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5670.11602827242701678No Hit
TAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTCCAAATTACCA5640.11541436622369923No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5610.11480046002038168No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5580.11418655381706415No Hit
CTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGC5540.11336801221264074No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCACCTTGAGTCCCTTTTTACC5520.11295874141042904No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5510.1127541060093232No Hit
GACCAATTTATGCCTACAGCCTCCTAAAACAAACAGTCTTAATCTAATCT5470.1119355644048998No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG5470.1119355644048998No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5440.11132165820158224No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5430.11111702280047638No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5430.11111702280047638No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5400.11050311659715885No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5350.10947993959162959No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5340.10927530419052374No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA5300.10845676258610035No Hit
ATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGTTTGGA5260.10763822098167695No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT5210.10661504397614768No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5210.10661504397614768No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5210.10661504397614768No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5200.10641040857504185No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTGAAGCATTCTTTGATTTTCTGT5170.10579650237172429No Hit
GTTATGTTAACACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTT5140.10518259616840676No Hit
CATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCT5130.1049779607673009No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5130.1049779607673009No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGGGCAG5120.10477332536619505No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5060.10354551295955995No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5060.10354551295955995No Hit
CTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGG5030.1029316067562424No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTC5000.10231770055292486No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4980.10190842975071315No Hit
CTCCGAAAGACACCAAATATTCTATGACCGTTTGTCTTCCAAAAGTGAGA4970.1017037943496073No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTATCGCACCTTGAG4960.10149915894850146No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4950.1012945235473956No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4950.1012945235473956No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4950.1012945235473956No Hit
GTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTCCAAATT4940.10108988814628976No Hit
TAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4910.1004759819429722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)