FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488674
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16770.34317356765450996No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACAGT15370.314524611499691No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14300.2926286235813651No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13340.2729836250752035No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12840.26275185501991105No Hit
GTATAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12510.255998886783418No Hit
CATTTAAACCCTCACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT11240.23001019084297508No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11230.22980555544186923No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10740.2197784207876826No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT10680.21855060838104748No Hit
TTCTATGACCGTTTGTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10330.21138836934234276No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9420.1927665478417104No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9240.18908311062180513No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9160.18744602741295832No Hit
CTTATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGTTT8850.181102329978677No Hit
GTCTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG8790.1798745175720419No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8390.17168910152780792No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8310.1700520183189611No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8170.1671871227034792No Hit
GTCATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8160.16698248730237336No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8040.16452686248910317No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA7780.15920634206035106No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA7710.15777389425261013No Hit
CTCTTATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGT7680.15715998804929257No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7630.1561368110437633No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA7390.15122556141722293No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7330.14999774901058785No Hit
CTTCTAGATACCGCCTCAGCTCTATTTCGGGAAGCCTTAGAGTCTCCTGA7140.1461096763895767No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7090.14508649938404744No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7060.14447259318072989No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6990.14304014537298892No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6920.14160769756524802No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6710.13731035414202516No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6660.1362871771364959No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6620.1354686355320725No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6540.13383155232322572No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6520.13342228152101399No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6500.1330130107188023No Hit
ATATAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGTTTGGA6470.13239910451548476No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6330.12953420890000286No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6320.129329573498897No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6290.12871566729557946No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG6220.12728321948783852No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6180.12646467788341512No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6130.1254415008778859No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6010.12298587606461567No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5980.12237196986129813No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5960.12196269905908642No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5920.12114415745466303No Hit
CTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGC5890.12053025125134548No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACATCCTTTGTTTA5890.12053025125134548No Hit
ATACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5880.12032561585023963No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5840.11950707424581623No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT5780.11827926183918112No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5730.1172560848336519No Hit
GACCAATTTATGCCTACAGCCTCCTAAAACAAACAGTCTTAATCTAATCT5680.11623290782812262No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5640.11541436622369923No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5610.11480046002038168No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5610.11480046002038168No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5600.11459582461927584No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5580.11418655381706415No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5580.11418655381706415No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA5520.11295874141042904No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCACCTTGAGTCCCTTTTTACC5520.11295874141042904No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5470.1119355644048998No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5460.11173092900379393No Hit
CCATAGAATGTATAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGAT5410.11070775199826469No Hit
CATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTTGTCT5310.10866139798720618No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT5230.1070243147783594No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5200.10641040857504185No Hit
GTTATGTTAACACTCATATGGGCCTAAAGTTCAGACAACTATTGTGGTTT5170.10579650237172429No Hit
CTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGG5140.10518259616840676No Hit
TAATATACCCGCCTTCCATAGAATGTATAAATAATGTCTAGTTTGGAAGT5110.10456868996508921No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5090.10415941916287749No Hit
AGTCTGTATCGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5070.10375014836066582No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5050.1033408775584541No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5050.1033408775584541No Hit
CTCCGAAAGACACCAAATATTCTATGACCGTTTGTCTTCCAAAAGTGAGA5040.10313624215734826No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4970.1017037943496073No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4960.10149915894850146No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4940.10108988814628976No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4940.10108988814628976No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTGAAA4920.10068061734407804No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTGAAGCATTCTTTGATTTTCTGT4920.10068061734407804No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTC4920.10068061734407804No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4920.10068061734407804No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTATCGCACCTTG4910.1004759819429722No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG4910.1004759819429722No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4890.10006671114076052No Hit
GTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTCCAAATT4890.10006671114076052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)