FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355153
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21410.6028387765272994No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGA17150.482890472556898No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTCCTAAC12520.3525241234059687No Hit
GTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGATGTTCTGGAT11020.31028880510653156No Hit
CATTTACACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCT10410.2931131089980938No Hit
TCCAGAGAGTGTGTAAATAGTGTCTGGTTTGAAAGTAATGATTAACTAGA10370.2919868338434421No Hit
GTTTAATACCCTTATCTAAGGGTAAATATTTGGTAAAGTTAGGGTAAAAC8890.25031465312133083No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC8030.22609973729632019No Hit
GTCTAGAAGATCCCGTACAGACGGAAAGAAATCAGAAGGCAAAAATGAGA7630.2148369857498036No Hit
GTTTGTAGGCCCTCTTACTGTCAATGAGAAAAGAAGATTGCAATTGATTA7370.20751619724456785No Hit
GTCTTTAACCCTCATTGGAAAACACCAACTTTTCCTAACATACATTTACA7000.19709815206404No Hit
CTCTTACTGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG6880.19371932660008503No Hit
GGGTAAAGGTTCAGGTATTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA6650.187243244460838No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6400.18020402474426514No Hit
ATCTAAGGGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGGCATAA6170.17372794260501812No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTC6150.1731648050276923No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC6120.17232009866170356No Hit
GTCCAAGAGTCCTCTTATGTAAGATCTTGGGCAAGATTTGGTGGGCGTTC6100.1717569610843777No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACC5900.16612558531111943No Hit
ATTCTACACAAGCTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGCC5830.16415460379047903No Hit
CCCTTAGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCA5670.1596495031718724No Hit
GTTCTGGATAGTAAGGTTTAATACCCTTATCTAAGGGTAAATATTTGGTA5620.15824165922855785No Hit
CCTACAAACTGTTCACATTTGTTGATGATGTCTTGGTGTAAATGTATGTT5600.157678521651232No Hit
CATTTAAACCCTAACAAGACGAAAAGATGGGGTTATTCTCTTCACTTCAT5540.15598910891925452No Hit
CCCTTATCTAAGGGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGG5360.15092087072332208No Hit
ATGTGGTATTGGGGGCCAAGTCTGTTCAGCATCTTGAGTCCCTTTTTACC5340.15035773314599624No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5340.15035773314599624No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCAA5240.1475420452593671No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5190.1461342013160525No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGTCCCACAATTCTTTGACATACT5180.14585263252738961No Hit
GGTATTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5090.14331851342942337No Hit
CTCCAAAACCGTGTCTCTCCCAAAAGTGAGACAAGAAATGTGAAACCACA5030.1416291006974459No Hit
CAGTAAGAGGGCCTACAAACTGTTCACATTTGTTGATGATGTCTTGGTGT4860.13684243129017637No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4810.13543458734686178No Hit
CTCTCTGGAAGGCGGGTATTTTATATAAGAGAGAAACAACACATAGCGCC4590.12924007399627765No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCAGCTACCAATCGCCAGTC4550.128113798841626No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTGACAGTAAGAGGGCCTAC4470.12586124853232267No Hit
GGATAGTAAGGTTTAATACCCTTATCTAAGGGTAAATATTTGGTAAAGTT4450.12529811095499685No Hit
GTGTAAATGTATGTTAGGAAAAGTTGGTGTTTTCCAATGAGGGTTAAAGA4440.12501654216633393No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGTCCCACAAT4440.12501654216633393No Hit
CTTCTAGACACCGCCGCAGCGCTATATCGGGATGCGTTAGAGTCTCCTGA4420.12445340458900812No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGCAGCA4420.12445340458900812No Hit
CTTTAACCCTCATTGGAAAACACCAACTTTTCCTAACATACATTTACACC4420.12445340458900812No Hit
CCTAATAACTCTATCTACCTGGGTGGGTGCTAATTTGGAAGATCCAGCAT4400.12389026701168229No Hit
GGGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGGCATAATCAATT4210.11854046002708692No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4210.11854046002708692No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCTAAAACATTCTTTCAGCTTGTGT4150.11685104729510942No Hit
GGGTAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCATTGAC4140.11656947850644651No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGATTTCTTTCCGTCTGTACG4120.11600634092912068No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT4090.11516163456313194No Hit
CATTCTACACAAGCTGAAAGAATGTTTTAGAAAACTCCCTGTTAACAGGC4070.11459849698580611No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA3950.11121967152185115No Hit
CACCAAGACATCATCAACAAATGTGAACAGTTTGTAGGCCCTCTTACTGT3940.11093810273318824No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3930.11065653394452532No Hit
AGATAAGGGTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACT3920.11037496515586241No Hit
CTCTTGGACTCTCCACACTGTCAACGACCGATCTTGAGCAATACTTCAAA3890.10953025878987367No Hit
GTTATGTCAACACTAACATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT3840.1081224148465591No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACTTTTGGGTTTT3820.10755927726923327No Hit
GTCAACGACCGATCTTGAGCAATACTTCAAAGACTGTTTGTTTAAGGACT3800.10699613969190744No Hit
GGTTTGTAGTATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGGCCTCCGT3770.10615143332591868No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3740.10530672695992994No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCG3720.10474358938260411No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCAACTTTTCCTAACATACA3670.10333574543928954No Hit
GTATTAAACCTTACTATCCAGAACATCTAGTTAATCATTACTTTCAAACCAGACACTATTTACACACTCTATGGA3620.10192790149597497No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTAGTCTCCTCC3620.10192790149597497No Hit
ATATTTACCCTTAGATAAGGGTATTAAACCTTACTATCCAGAACATCTAG3610.10164633270731205No Hit
AATGTGAACAGTTTGTAGGCCCTCTTACTGTCAATGAGAAAAGAAGATTG3600.10136476391864914No Hit
GGTAAATATTTGGTAAAGTTAGGGTAAAACCTAGCAGGCATAATCAATTG3580.10080162634132331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)