FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589946
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62521.0597580117502279No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTGTGGAAGTAATGATTAACTAGA43230.7327789323090588No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT19380.3285046427978154No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTGTGGA17560.2976543615856367No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT16540.2803646435436464No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC16180.27426239011706155No Hit
GTGTAAATAATGTCTGGTGTGGAAGTAATGATTAACTAGATGTTCTGGAT14700.24917534825221294No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT14640.24815830601444877No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG14510.24595471449929315No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT13700.23222464428947734No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13310.2256138697440105No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13300.2254443627043831No Hit
GTACACCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC13230.2242578134269916No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC12510.212053306573822No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCACACC11630.1971366870866147No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10440.17696534937095937No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC10430.17679584233133203No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT10230.1734057015387849No Hit
CTCTACGACCGTATCTCTTCCGAAAGTGAGACAAGAAATGTGAAACCACA10150.17204964522176605No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT9870.1673034481122001No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC9770.16560837771592654No Hit
CACTTACAGTTAATGAGAAGAGAAGACTGCAATTGATTATGCCTGCTAGG9170.1554379553382852No Hit
ATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGTTAATGAGAAGAG9060.1535733779023843No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTTACAGT9040.15323436382312958No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8910.15103077230797396No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTGT8570.14526753296064385No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA8350.141538378088842No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7950.13475809650374782No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7530.12763880083939888No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCACACCAGACATTATT7460.12645225156200737No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7300.12374013892796967No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT7180.12170605445244141No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC7060.11967196997691315No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA7040.11933295589765844No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6930.11746837846175753No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTCTCA6920.11729887142213016No Hit
AATATACATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGG6910.1171293643825028No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCT6770.11475626582771983No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCACACCAGACATTATTTACACACTCTATGGA6750.11441725174846512No Hit
CTTACAGTTAATGAGAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTT6730.11407823766921041No Hit
GGCATAATCAATTGCAGTCTTCTCTTCTCATTAACTGTAAGTGGGCCTAC6680.11323070247107363No Hit
GTTAATGAGAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC6650.11272218135219154No Hit
GGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTCTTCTCATTAAC6650.11272218135219154No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTT6460.1095015475992718No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT6460.1095015475992718No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC6460.1095015475992718No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG6320.1071284490444888No Hit
CTACAAACCATGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6250.10594189976709734No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6230.10560288568784261No Hit
GTGTGTAAATAATGTCTGGTGTGGAAGTAATGATTAACTAGATGTTCTGG6220.10543337864821525No Hit
GAAGAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA6160.10441633641045112No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6080.10306028009343228No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG6020.10204323785566814No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCACA5980.10136520969715873No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5950.10085668857827666No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTGTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC5940.1006871815386493No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC5940.1006871815386493No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC5900.10000915338013988No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG5900.10000915338013988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)